Nature Methods
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Papers 5,341
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#1Emmanuel Moebel (PSL: PSL Research University)H-Index: 5
#2Antonio Martinez-Sanchez (GAU: University of Göttingen)H-Index: 12
Last. Charles Kervrann (PSL: PSL Research University)H-Index: 2
view all 14 authors...
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#1Jeff Demas (Rockefeller University)H-Index: 9
#2Jason Manley (Rockefeller University)H-Index: 5
Last. Alipasha Vaziri (Rockefeller University)H-Index: 33
view all 8 authors...
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#1Claire Walsh (UCL: University College London)H-Index: 14
#2Paul Tafforeau (ESRF: European Synchrotron Radiation Facility)H-Index: 56
Last. Peter D. Lee (UCL: University College London)H-Index: 81
view all 18 authors...
Imaging intact human organs from the organ to the cellular scale in three dimensions is a goal of biomedical imaging. To meet this challenge, we developed hierarchical phase-contrast tomography (HiP-CT), an X-ray phase propagation technique using the European Synchrotron Radiation Facility (ESRF)'s Extremely Brilliant Source (EBS). The spatial coherence of the ESRF-EBS combined with our beamline equipment, sample preparation and scanning developments enabled us to perform non-destructive, three-...
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The proliferation of versatile open software and hardware for microscopy is helping to democratize biological imaging for both current and aspiring scientists.
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#1Kishwar Shafin (UCSC: University of California, Santa Cruz)H-Index: 7
#2Trevor Pesout (UCSC: University of California, Santa Cruz)H-Index: 7
Last. Karen H. Miga (UCSC: University of California, Santa Cruz)H-Index: 25
view all 14 authors...
Long-read sequencing has the potential to transform variant detection by reaching currently difficult-to-map regions and routinely linking together adjacent variations to enable read-based phasing. Third-generation nanopore sequence data have demonstrated a long read length, but current interpretation methods for their novel pore-based signal have unique error profiles, making accurate analysis challenging. Here, we introduce a haplotype-aware variant calling pipeline, PEPPER-Margin-DeepVariant,...
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#1Ian Walsh (A*STAR: Agency for Science, Technology and Research)H-Index: 22
#2Dmytro Fishman (UT: University of Tartu)H-Index: 6
view all 36 authors...
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The MISpheroID knowledgebase records and organizes experimental parameters from thousands of cancer spheroid experiments, revealing heterogeneity and a lack of transparency in key spheroid research reporting practices.
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#1Shaina Lu (CSHL: Cold Spring Harbor Laboratory)H-Index: 2
#2Daniel Fürth (CSHL: Cold Spring Harbor Laboratory)H-Index: 8
Last. Jesse Gillis (CSHL: Cold Spring Harbor Laboratory)H-Index: 33
view all 3 authors...
Computational methods use different integrative strategies to tackle the challenges of spatially resolved transcriptomics data analysis.
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#1Arne Peirsman (Ghent University)H-Index: 1
#2Eva Blondeel (Ghent University)H-Index: 1
Last. Flávia Castro (University of Porto)H-Index: 8
view all 51 authors...
Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase that assembles the experimental parameters of 3,058 published spheroid-related experiments. Interrogation of this knowledgebase identified heterogeneity in the methodological setup of spheroids. Empi...
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