Cell seeding simulation on micropatterned islands shows cell density depends on area to perimeter ratio, not on island size or shape

Protein micropatterned substrates have been used to control cell size, shape, and cell–cell contacts, characteristics that influence a range of cell behaviors from early cell differentiation to late stages of maturation. Knowing the initial island cell seeding density is important to interpreting results and understanding downstream cell behavior. While studies routinely report the intended or target cell seeding density, they do not report the...