Indirectly stimulation of DCs by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model based on RNA-seq

Published on Apr 1, 2020in Journal of Functional Foods3.701
· DOI :10.1016/J.JFF.2020.103850
Xiaomeng Ding3
Estimated H-index: 3
(NCU: Nanchang University),
Qiang Yu21
Estimated H-index: 21
(NCU: Nanchang University)
+ 6 AuthorsMingyong Xie71
Estimated H-index: 71
(Jiangxi Normal University)
Abstract Our previous study has demonstrated that Ganoderma atrum polysaccharide (PSG-1) activated the immune response of dendritic cells (DCs) by direct contacting with surface receptors. However, as a kind of macromolecule compound, PSG-1 cannot directly stimulate DCs via passing through the intestine epithelial cells. To investigate the role of PSG-1 in the interaction between the intestinal epithelium and its internal immune cells, the intestinal-like Caco-2/DCs co-culture model was established, then all transcripts of DCs with or without treatment of PSG-1 were globally screened by RNA-seq. According to the RNA-seq analysis, cytokine receptor interaction, NF-κB and MAPK signaling pathway were three important signal pathways annotate to the environmental information processing. Moreover, cytokine and related proteins expression were in DCs indirectly stimulated by PSG-1 were raised. These above findings indicated that PSG-1 indirectly affected the immune function of DCs in intestinal-like Caco-2/DCs co-culture model, in which NF-κB and MAPK pathway played essential roles.
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