Cucurbitacin D Overcomes Gefitinib Resistance by Blocking EGF Binding to EGFR and Inducing Cell Death in NSCLCs

In this study, the mechanism of the anti-cancer effect through which cucurbitacin D (CuD) can overcome gefitinib resistance in NSCLC was investigated. Cell viability was measured by MTT assay, and cell migration and growth were observed by wound-healing and colony formation assays, respectively. Levels of EGFR family members, AKT, ERK, PARP and G2/M phase-related proteins were detected by western blot analysis. Immunofluorescence analysis was...