polysaccharides

galactose

arabinose

d-glc

sulfated

polysaccharide_56168

d-gal

antioxidant activities

International Journal of Biological Macromolecules

A water-soluble heteropolysaccharide was isolated and purified from Enteromorpha prolifera by DEAE-52 and Bio-Gel P-2 column chromatography . Fourier transform infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), multi-angle laser light scattering (MALLS), and nuclear magnetic resonance (NMR) spectroscopy were used to characterize the structure of E. prolifera polysaccharide degradation (EPP-1). Its anti-oxidative activity was determined in Caenorhabditis elegans via modulation of microRNAs . The average molecular weight of EPP-1 was 4.28 kDa. It contained six types of linkage units as →2)-β- d -Glc p A-(1→, →3,6)-β- d -Man p -(1→, →4)-α- d -Glc p -(1→, →6)-β- d -Gal p -(1→, β- l -Rha p -(1→, and →4)-β- d -Gal p A-(1→. The mean lifespan, ultraviolet-induced oxidative stress , and thermotolerance in C. elegans were improved after treatment of EPP-1. Moreover, EPP-1 significantly increased the total superoxide dismutase levels and decreased the malondialdehyde levels in C. elegans . Intracellular reactive oxygen species accumulation and DNA damage were ameliorated by up-regulation of SKN-1 and DAF-16 expression through miR-48 and miR-51 miR-186 down-regulation. In vivo studies demonstrated that EPP-1 might be applied in functional foods as the antioxidative and anti-ageing ingredient.

Structural characterization and antioxidant effect of green alga Enteromorpha prolifera polysaccharide in Caenorhabditis elegans via modulation of microRNAs