Bioconversion of rice straw by synergistic effect of in-house produced ligno-hemicellulolytic enzymes for enhanced bioethanol production

Published on Jun 1, 2020in Bioresource Technology Reports
· DOI :10.1016/J.BITEB.2019.100352
Nisha Bhardwaj9
Estimated H-index: 9
(Central University of Rajasthan),
Bikash Kumar10
Estimated H-index: 10
(Central University of Rajasthan)
+ 1 AuthorsPradeep Verma23
Estimated H-index: 23
(Central University of Rajasthan)
Sources
Abstract
Abstract In the present work attempts have been made to mimic the natural system of biomass delignification using laccase from Myrothecium verrucaria ITCC-8447 and synergistic effect of cellulase and xylanase from Schizophyllum commune NAIMCC-F-03379 and Aspergillus oryzae ITCC-8571 respectively for enhanced saccharification and subsequent bioethanol production. The laccase mediated pretreatment resulted in 6.7% delignification of rice straw (RS). The structural analysis of enzymatic pretreated RS using XRD and FTIR suggested high crystallinity index (CrI) and improved accessibility of cellulose and hemicelluloses as compared to untreated RS. The single step simultaneous delignification, saccharification and fermentation (SDSF) of RS using partially purified in-house enzyme cocktail with Saccharomyces cerevisiae MTCC-173 resulted in sugar yield of 26.7 ± 0.2 g/L with an experimental ethanol yield of 6.47 ± 0.16 g/L which is comparable to maximum sugar and ethanol yield of 31.2 ± 0.7 g/L and 7.34 ± 0.39 g/L respectively using commercial enzyme cocktail with S. cerevisiae MTCC-173. The SDSF was obtained as a suitable single step process for the ethanol production from RS.
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References47
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#1Bikash Kumar (Central University of Rajasthan)H-Index: 10
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Abstract In this study, Box–Behnken design Response Surface Methodology (BBD-RSM) was used to develop microwave-assisted pretreatment (MAP) for Rice Straw (RS) using FeCl3-H3PO4 system. The concentration of FeCl3 and H3PO4, pretreatment temperature and time was used as independent variables and complex severity considering the effect of all variables were also calculated. Based on BBD-RSM, 57.5% of sugar yield per biomass was obtained for pretreated (MAP) RS using FeCl3 (0.35 M), H3PO4 (3%), pre...
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Abstract The white laccase was produced from Myrothecium verrucaria ITCC-8447 under submerged fermentation. The media components were optimized by response surface methodology (CCD-RSM). The nutritional components (glucose and peptone) and physical parameters (pH and temperature) were optimized by response surface methodology for enhanced laccase production by Myrothecium verrucaria ITCC-8447. The enzyme activity under optimum condition exhibited 1.45 fold increases in laccase activity. The whit...
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Abstract Xylooligosaccharides (XOS) from lignocellulosic biomass (LCB) have found widespread applications in food, feed, nutraceuticals and pharamecutical industries. Enzymatic degradation of LCB for generation of XOS have gained impetus in recent times In the present investigation an extracellular thermo-alkali stable xylanase from Aspergillus oryzae LC1 was purified by using PEG 8000/MgSO 4 aqueous two-phase system and was capable of hydrolysing various agricultural residues into XOS system. H...
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A cellulase producing fungus Schizophyllum commune NAIMCC-F-03379 was isolated from decomposed leaf sample of Lantana camera. The nutritional components (wheat bran, magnesium sulphate and calcium chloride concentrations) and physical parameters (temperature and pH) were optimised by response surface methodology for enhanced cellulase production by S. commune NAIMCC-F-03379. The optimized medium contained: 1% (w/v) wheat bran, 0.3 g/L MgSO4, 0.8–1.0 g/L CaCl2, optimum temperature and pH were 25 ...
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Advanced techniques of enzyme production and purification have become prerequisite due to their diverse industrial applications. There is an utmost requirement for screening of new strains capable of synthesising industrially useful enzymes. The present study reports the production and profiling of extracellular proteins expressed by the newly isolated strain of a filamentous fungus, Aspergillus oryzae LC1. The extracellular enzyme production was done by submerged fermentation using Mendel’s and...
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