Determination of seven oligosaccharides and sucrose in Pseudostellaria heterophylla by pressurized liquid extraction and ultra-high performance liquid chromatography with charged aerosol detector and tandem mass spectrometry

A simple, rapid, and sensitive ultra-high performance liquid chromatography with charged aerosol detector (UPLC-CAD) method was developed for firstly simultaneous determination of seven oligosaccharides, including two pairs of linear oligosaccharides isomers (DP3-1, DP3-2 and DP4-1, DP4-2) and 3 high branched oligosaccharides (DP 5, 6 and 7), as well as sucrose in Pseudostellaria heterophylla. The separation was performed on a Waters BEH Amide...