Purpose: We evaluate the in vitro antibacterial activity of silver-copolymerized barrel cases compared to nonsilver barrel cases in combination with a multipurpose disinfecting solution (MPDS). Methods: The ability of silver and nonsilver cases to reduce the number of adherent Gram-positive and Gram-negative bacteria was assessed. Additionally, the efficacy of silver cases along with MPDS was investigated in the presence of organic soil and contact lenses. Contact lens cases were challenged with 106 colony-forming units (CFU)/mL of five bacterial species. Adherent bacteria were dislodged from lens cases and surviving organisms enumerated. Results: Significantly lower numbers of microbes were recovered from silver cases compared to controls, for all bacterial strains (P < 0.005). The combination of silver case along with MPDS showed added efficacy against Gram-positive and -negative bacteria with a maximum reduction of 3.00 ± 0.5 Log10 CFU/mL, compared to the efficacy of silver cases alone (1.97 ± 0.4 Log10 CFU/mL). The addition of organic soil and a contact lens resulted in a significant (P < 0.005) decrease (a maximum of 1.68 ± 0.2 log10 CFU/mL) in disinfection efficacy when MPDS and either silver or control cases were used. Conclusions: Silver-copolymerized barrel cases work on conjunction with a hypochlorite producing MPDS in the presence of contact lenses and organic soil to reduce microbial contamination of lens cases. Transitional Relevance: Silver-copolymerized barrel contact lens cases show promising in vitro antibacterial activity against bacterial types commonly implicated in contact lens-related corneal infections. This intervention may limit storage case contamination during use and reduce the frequency of contact lens-related microbial disease.
The aim of this study was to use antimicrobial efficacy endpoint methodology to determine compatibility of multipurpose disinfecting solutions (MPSs), lens cases, and hydrogel lenses for disinfection (AEEMC) against International Organization for Standardization (ISO)–specified microorganisms and clinical ocular isolates of Stenotrophomonas maltophilia. Six MPSs (PQ/Aldox 1, 2, and 3; PQ/Alexidine; PQ/PHMB; and PHMB) were challenged against ISO-specified microorganisms and S. maltophilia using t...
Last. Fiona Stapleton(UNSW: University of New South Wales)H-Index: 67
view all 4 authors...
A contaminated contact lens case can act as a reservoir for microorganisms that could potentially compromise contact lens wear and lead to sight threatening adverse events. The rate, level and profile of microbial contamination in lens cases, compliance and other risk factors associated with lens case contamination, and the challenges currently faced in this field are discussed. The rate of lens case contamination is commonly over 50%. Coagulase-negative Staphylococcus, Bacillus spp., Pseudomona...
Silver nanoparticles (AgNPs) have been used as antibacterial, antifungal, antiviral, anti-inflammtory, and antiangiogenic due to its unique properties such as physical, chemical, and biological properties. The present study was aimed to investigate antibacterial and anti-biofilm activities of silver nanoparticles alone and in combination with conventional antibiotics against various human pathogenic bacteria. Here, we show that a simple, reliable, cost effective and green method for the synthesi...
#1Shima Taheri(UniSA: University of South Australia)H-Index: 16
#2Alex Cavallaro(UniSA: University of South Australia)H-Index: 18
Last. Krasimir Vasilev(UniSA: University of South Australia)H-Index: 54
view all 8 authors...
Abstract Infections arising from bacterial adhesion and colonization on medical device surfaces are a significant healthcare problem. Silver based antibacterial coatings have attracted a great deal of attention as a potential solution. This paper reports on the development of a silver nanoparticles based antibacterial surface that can be applied to any type of material surface. The silver nanoparticles were surface engineered with a monolayer of 2-mercaptosuccinic acid, which facilitates the imm...
Last. Branimir Cerovski(University Hospital Centre Zagreb)H-Index: 6
view all 8 authors...
Abstract Purpose This study aims to investigate the association of compliance amongst lens wearers and lens case contamination as assessed by the microbial profile of lens cases. Methods Fifty-two asymptomatic lens wearers filled out questionnaires seeking demographic data and several aspects of compliance to lens wear. Subsequently, contamination profiles of the inside bottom and rim of their lens cases was obtained for bacteria, fungi and Acanthamoeba. The association of the self-reported resp...
• Rates for CIE, SICS and GNB case contamination for four LCPs when used with balafilcon A (Bausch + Lomb, Rochester, USA) were obtained either from published data or calculated retrospectively. • The four LCPs were o LCP 1 containing sodium chlorite / H2O2: Sauflon Pharmaceuticals, Twickenham, UK. o LCP 2 containing alexidine dihydrochloride / polyquaternium (PQ)-1: Abbott Medical Optics, Inc., Santa Ana, USA. o LCP 3 containing PHMB / PQ-1: Bausch + Lomb, Rochester, USA. o LCP 4 containing PHM...
OBJECTIVE: To investigate the effects of eight different soft contact lenses on disinfection efficacy of a multipurpose solution (MPS) containing polyhexamethylene biguanide (PHMB) against Fusarium solani. METHODS: Six silicone hydrogel lenses (galyfilcon A, senofilcon A, comfilcon A, enfilcon A, balafilcon A, and lotrifilcon B) and two conventional hydrogel lenses (polymacon and etafilcon A) were placed in polypropylene lens cases filled with MPS containing 0.0001% PHMB and soaked for 6, 12, 24...
#1Jaya Dantam(UNSW: University of New South Wales)H-Index: 3
#1Jaya Dantam(UNSW: University of New South Wales)H-Index: 2
Last. Fiona Stapleton(UNSW: University of New South Wales)H-Index: 67
view all 7 authors...
PURPOSE: To evaluate microbial contamination in silver-impregnated contact lens (CL) storage cases while establishing the effect of "wet " and "dry " case maintenance and to determine its association with clinical signs, symptoms, and compliance. METHODS: Two noncontemporaneous prospective studies were conducted. Regular storage cases in study 1 (n = 40) and silver-impregnated cases in study 2 (n = 41) were used in conjunction with CL solution and CLs (balafilcon A). Cases were replaced monthly ...
Last. Radek Zboril(Palacký University, Olomouc)H-Index: 78
view all 3 authors...
Abstract Utilization of metallic nanoparticles in various biotechnological and medical applications represents one of the most extensively investigated areas of the current materials science. These advanced applications require the appropriate chemical functionalization of the nanoparticles with organic molecules or their incorporation in suitable polymer matrices. The intensified interest in polymer nanocomposites with silver nanoparticles is due to the high antimicrobial effect of nanosilver a...
Contact lenses in the future will likely have functions other than correction of refractive error. Lenses designed to control the development of myopia are already commercially available. Contact lenses as drug delivery devices and powered through advancements in nanotechnology will open up further opportunities for unique uses of contact lenses. This review examines the use, or potential use, of contact lenses aside from their role to correct refractive error. Contact lenses can be used to dete...
Last. Lyndon Jones(UW: University of Waterloo)H-Index: 69
view all 11 authors...
Contact lens materials have undergone significant changes over the past 20 years, particularly with respect to the introduction of silicone hydrogel materials. Whilst this development addressed hypoxic issues, other important areas relating to contact lens success, notably comfort, require further research. Contact lens wettability remains a crucially important part of biocompatibility. Contact lenses can be made more wettable by incorporation of surfactants into blister packs, internal wetting ...
Last. Prashant Garg(LVPEI: L V Prasad Eye Institute)H-Index: 36
view all 5 authors...
Purpose To evaluate the efficacy of a nanosilver-based sol-gel coating in preventing biofilm formation on contact lens cases. Methods An organic-inorganic hybrid silica-zirconia sol formulation with immobilized silver nanoparticles was deposited on contact lens case coupons. The coated and uncoated coupons were subjected to biofilm formation to Gram-negative and Gram-positive keratitis isolates and ATCC strains using a standard protocol. The biofilms were evaluated using crystal violet, MTT assa...
#1Ajay Kumar Vijay(UNSW: University of New South Wales)H-Index: 11
#2Hua Zhu(UNSW: University of New South Wales)H-Index: 25
Last. Fiona Stapleton(UNSW: University of New South Wales)H-Index: 67
view all 5 authors...
Abstract Purpose This study investigated the efficacy of pre-conditioning lens cases on bacterial biofilm formation and removal. Methods Silver impregnated (MicroBlock / ProGuard™ & i-Clean) and control storage cases were pre-conditioned for 24 h with their respective multipurpose solutions (MPDSs). Cases were then inoculated with 2 ml of 106 CFU/mL of ocular isolates of either P. aeruginosa or S. aureus and incubated for 48 h. Cases were subsequently disinfected (4−6 hours) as per the manufactu...
#1Fiona Stapleton(UNSW: University of New South Wales)H-Index: 67
Microbial keratitis is a rare but potentially severe sight-threatening condition, associated with societal burden, cost and morbidity. Compared with microbial keratitis without lens wear, the disease in contact lens wear is more common, occurs at an earlier age, has lower morbidity and is more often caused by Pseudomonas aeruginosa and Acanthamoeba spp. Resistance to common antibiotics is infrequent in contact lens-related isolates and there is little evidence to suggest increasing bacterial res...