Improved quantitative detection of biotin‐labeled red blood cells by flow cytometry

BACKGROUND Biotin‐labeled red blood cells (BioRBC) can be tracked after transfusion, providing a convenient and safe way to measure RBC survival in vivo. RBC survival is of interest for determining optimal blood storage conditions and for assessing the impact of genetic and biologic variants in blood donors on the survival of transfused RBCs. Here we present an improved, platform‐independent assay for quantifying biotin on BioRBC. This approach...