Determination and tissue distribution studies of dantrolene sodium with hydroxypropyl‐ β ‐cyclodextrin in rat tissue by HPLC/MS/MS

The established analytical method for determining the concentration of dantrolene sodium (Da) in rat tissues by HPLC/MS/MS technique was successfully applied to tissue distribution studies of Da in rats. Tissue homogenate samples were pretreated by protein precipitation with pre‐cooled methanol. Chromatographic separation was achieved on an Acquity HPLC column (Kromat Universil XB‐C 18 , 2.1 × 150 mm, 3 μm). Mass spectrometry was conducted with...