Current good manufacturing practices-compliant manufacture and measurement of biotin-labeled red blood cells.

Published on Jul 1, 2019in Cytotherapy4.218
· DOI :10.1016/J.JCYT.2019.04.052
Albert D. Donnenberg27
Estimated H-index: 27
(University of Pittsburgh),
Tamir Kanias23
Estimated H-index: 23
+ 7 AuthorsMark T. Gladwin122
Estimated H-index: 122
(University of Pittsburgh)
Abstract Background Red blood cells (RBCs) can be labeled with N-hydroxysuccinimidobiotin (sulfo-NHS-biotin), which binds to cell surface proteins under aqueous conditions. Biotinylated RBCs can be safely infused and detected in peripheral blood samples using flow cytometry, using a fluorochrome-conjugated streptavidin (SA) detection reagent. Biotinylated RBCs have been used to track survival of transfused RBCs, and have applications in optimizing RBC storage and in understanding donor genetic, environmental and disease factors affecting RBC products. Methods We have developed a closed-system, current good manufacturing practices (cGMP)–compliant procedure for biotinylation of RBCs and a quantitative flow cytometric assay to estimate the dose of cell-bound biotin delivered to the patient. Resulting products were characterized for variability, sterility, endotoxin, hemolysis, total dose of cell-bound biotin and stability. Results The density of biotin-labeling increased as a log-linear function of sulfo-NHS-biotin–labeling concentration, with greater variability at lower concentrations. The upper estimates of biotin doses in the average product (mean RBC content = 5.55 × 1011) were 9.8 and 73.0 µg for products labeled at 3 and 15 µg sulfo-NHS-biotin/mL of total reaction mixture (27 and 135 nmol/mL packed RBCs), respectively. All products were negative for bacterial and fungal growth at 14 days and were below the limit of endotoxin detection. Biotinylated RBCs were stable in vitro for up to 50 days after labeling. Discussion We have validated a closed-system procedure for biotinylating RBCs for investigational use. A standard operating procedure is presented in sufficient detail for implementation in a cGMP-compliant cell-processing facility.
📖 Papers frequently viewed together
1 Citations
1 Author (OF Light)
7 Citations
#1Marion C. Lanteri (USF: University of San Francisco)H-Index: 27
#2Tamir KaniasH-Index: 23
Last. Michael P. Busch (USF: University of San Francisco)H-Index: 110
view all 18 authors...
BACKGROUND: Genetic determinants may underlie the susceptibility of red blood cells (RBCs) to hemolyze in vivo and during routine storage. This study characterized the reproducibility and dynamics of in vitro hemolysis variables from a subset of the 13,403 blood donors enrolled in the RBC-Omics study. STUDY DESIGN AND METHODS: RBC-Omics donors with either low or high hemolysis results on 4°C-stored leukoreduced (LR)-RBC samples from enrollment donations stored for 39 to 42 days were recalled 2 t...
28 CitationsSource
#1Angelo D'Alessandro (University of Colorado Denver)H-Index: 67
#2Rachel Culp-Hill (University of Colorado Denver)H-Index: 14
Last. James C. ZimringH-Index: 35
view all 17 authors...
BACKGROUND: Biological and technical variability has been increasingly appreciated as a key factor impacting red blood cell (RBC) storability and, potentially, transfusion outcomes. Here, we performed metabolomics analyses to investigate the impact of factors other than storage duration on the metabolic phenotypes of stored RBC in a multicenter study. STUDY DESIGN AND METHODS: Within the framework of the REDS-III (Recipient Epidemiology and Donor Evaluation Study-III) RBC-Omics study, 13,403 don...
39 CitationsSource
#1Tamir Kanias (University of Pittsburgh)H-Index: 23
#2Mars Stone (UCSF: University of California, San Francisco)H-Index: 26
Last. Alan E. Mast (MCW: Medical College of Wisconsin)H-Index: 36
view all 15 authors...
BACKGROUND: Frequent whole blood donations increase the prevalence of iron depletion in blood donors, which may subsequently interfere with normal erythropoiesis. The purpose of this study was to evaluate the associations between donation frequency and red blood cell (RBC) storage stability in a racially/ethnically diverse population of blood donors. STUDY DESIGN: Leukoreduced RBC concentrate-derived samples from 13,403 donors were stored for 39 to 42 days (1-6°C) and then evaluated for storage,...
25 CitationsSource
#1Djuna Z. de Back (UvA: University of Amsterdam)H-Index: 4
#2Richard Vlaar (UvA: University of Amsterdam)H-Index: 3
Last. Robin van Bruggen (UvA: University of Amsterdam)H-Index: 26
view all 9 authors...
Several circumstances require the accurate measurement of red blood cell (RBC) survival and clearance, such as determination of posttransfusion recovery of stored RBCs to investigate the effect of new additive solutions. To this end, biotin as a marker of RBCs to track donor RBCs in the blood of the recipient has been used in many studies. However, so far only experimental, nonvalidated, biotin-labeled red cell concentrates (RCCs) are transfused. The goal of this study was to produce a standardi...
7 CitationsSource
#1Tamir Kanias (University of Pittsburgh)H-Index: 23
#2Marion C. Lanteri (Systems Research Institute)H-Index: 27
Last. Mark T. Gladwin (University of Pittsburgh)H-Index: 122
view all 15 authors...
Genetic polymorphisms in blood donors may contribute to donor-specific differences in the survival of red blood cells (RBCs) during cold storage and after transfusion. Genetic variability is anticipated to be high in donors with racial admixture from malaria endemic regions such as Africa and Asia. The purpose of this study was to test the hypothesis that donor genetic background, reflected by sex and self-reported ethnicity, significantly modulates RBC phenotypes in storage. High throughput hem...
83 CitationsSource
#1Robert L. Schmidt (Roy J. and Lucille A. Carver College of Medicine)H-Index: 20
#2Donald M. Mock (University of Arkansas for Medical Sciences)H-Index: 45
Last. John A. Widness (Roy J. and Lucille A. Carver College of Medicine)H-Index: 58
view all 11 authors...
Biotin-labeled red blood cells (BioRBCs) are used for in vivo kinetic studies. Because BioRBC dosing occasionally induces antibodies, a sensitive and specific anti-BioRBC detection assay is needed. Aims were to 1) develop a gel card assay to evaluate existing, naturally occurring and BioRBC-induced plasma antibodies, 2) compare gel card and tube agglutination detection results, and 3) test for a relationship of antibody induction and BioRBC dose. Reagent BioRBCs were prepared using sulfo-NHS bio...
11 CitationsSource
: Hemolysis is a fundamental feature of sickle cell anemia that contributes to its pathophysiology and phenotypic variability. Decompartmentalized hemoglobin, arginase 1, asymmetric dimethylarginine, and adenine nucleotides are all products of hemolysis that promote vasomotor dysfunction, proliferative vasculopathy, and a multitude of clinical complications of pulmonary and systemic vasculopathy, including pulmonary hypertension, leg ulcers, priapism, chronic kidney disease, and large-artery isc...
245 CitationsSource
#1Denison Kuruvilla (UI: University of Iowa)H-Index: 6
#2John A. Widness (UI: University of Iowa)H-Index: 58
Last. Peter Veng-Pedersen (UI: University of Iowa)H-Index: 30
view all 7 authors...
Estimation of adult and neonatal RBC lifespans in anemic neonates using RBCs labeled at several discrete biotin densities
7 CitationsSource
#1Tamir Kanias (University of Pittsburgh)H-Index: 23
#2Derek Sinchar (University of Pittsburgh)H-Index: 7
Last. Mark T. Gladwin (University of Pittsburgh)H-Index: 122
view all 10 authors...
BACKGROUND Red blood cell (RBC) hemolysis represents an intrinsic mechanism for human vascular disease. Intravascular hemolysis releases hemoglobin and other metabolites that inhibit nitric oxide signaling and drive oxidative and inflammatory stress. Although these pathways are important in disease pathogenesis, genetic and population modifiers of hemolysis, including sex, have not been established. STUDY DESIGN AND METHODS We studied sex differences in storage or stress-induced hemolysis in RBC...
73 CitationsSource
#1David O Osei-Hwedieh (University of Pittsburgh)H-Index: 5
#2Tamir Kanias (University of Pittsburgh)H-Index: 23
Last. Mark T. Gladwin (University of Pittsburgh)H-Index: 122
view all 18 authors...
Abstract Background Transfusion of blood at the limits of approved storage time is associated with lower red blood cell (RBC) post-transfusion recovery and hemolysis, which increases plasma cell-free hemoglobin and iron, proposed to induce endothelial dysfunction and impair host defense. There is noted variability among donors in the intrinsic rate of storage changes and RBC post-transfusion recovery, yet genetic determinants that modulate this process are unclear. Methods We explore RBC storage...
23 CitationsSource
Cited By3
#1Catherine Ravanat (UDS: University of Strasbourg)H-Index: 24
#2Anaïs Pongerard (UDS: University of Strasbourg)H-Index: 1
Last. Christian Gachet (UDS: University of Strasbourg)H-Index: 84
view all 10 authors...
BACKGROUND The production of platelet concentrates (PCs) is evolving, and their survival capacity needs in vivo evaluation. This requires that the transfused platelets (PLTs) be distinguished from those of the recipient. Labeling at various biotin (Bio) densities allows one to concurrently trace multiple PLT populations, as reported for red blood cells. STUDY DESIGN AND METHODS A method is described to label human PLTs at two densities of Bio for future clinical trials. Injectable-grade PLTs wer...
#1Nareg Roubinian (UCSF: University of California, San Francisco)H-Index: 22
#2Tamir KaniasH-Index: 23
1 CitationsSource
#1Albert D. Donnenberg (University of Pittsburgh)H-Index: 27
#2Tamir KaniasH-Index: 23
Last. Mark T. Gladwin (University of Pittsburgh)H-Index: 122
view all 6 authors...
BACKGROUND: Biotin-labeled red blood cells (BioRBC) can be tracked after transfusion, providing a convenient and safe way to measure RBC survival in vivo. RBC survival is of interest for determining optimal blood storage conditions and for assessing the impact of genetic and biologic variants in blood donors on the survival of transfused RBCs. Here we present an improved, platform-independent assay for quantifying biotin on BioRBC. This approach is also useful for detecting BioRBC in peripheral ...
4 CitationsSource