Current good manufacturing practices–compliant manufacture and measurement of biotin-labeled red blood cells
Abstract
Red blood cells (RBCs) can be labeled with N-hydroxysuccinimidobiotin (sulfo-NHS-biotin), which binds to cell surface proteins under aqueous conditions. Biotinylated RBCs can be safely infused and detected in peripheral blood samples using flow cytometry, using a fluorochrome-conjugated streptavidin (SA) detection reagent. Biotinylated RBCs have been used to track survival of transfused RBCs, and have applications in optimizing RBC storage and...
Paper Details
Title
Current good manufacturing practices–compliant manufacture and measurement of biotin-labeled red blood cells
Published Date
Jul 1, 2019
Journal
Volume
21
Issue
7
Pages
793 - 800
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Notes
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