Effective control of biofilms by photothermal therapy using a gold nanorod hydrogel.

Published on Feb 1, 2020in Journal of Biomedical Materials Research Part B2.831
· DOI :10.1002/JBM.B.34392
Carlos Bermúdez‐Jiménez2
Estimated H-index: 2
(Universidad Autónoma de San Luis Potosí),
Nereyda Niño-Martínez12
Estimated H-index: 12
(Universidad Autónoma de San Luis Potosí)
+ 4 AuthorsGabriel Alejandro Martínez-Castañón19
Estimated H-index: 19
(Universidad Autónoma de San Luis Potosí)
: Biofilms are matrices synthesized by bacteria containing polysaccharides, DNA, and proteins. The development of biofilms in infectious processes can induce a chronic inflammatory response that may progress to the destruction of tissues. The treatment of biofilms is difficult because they serve as a bacterial mechanism of defense and high doses of antibiotics are necessary to treat these infections with limited positive results. It has been demonstrated that photothermal therapy using gold nanorods (AuNRs) is an attractive treatment because of its anti-biofilm activity. The purpose of this work was to generate a novel chitosan-based hydrogel embedded with AuNRs to evaluate its anti-biofilm activity. AuNRs were synthesized by the seed-mediated growth method and mixed with the chitosan-based hydrogel. Hydrogels were characterized and tested against two bacterial strains by irradiating the produced biofilm in the presence of the nanoformulation with a laser adjusted at the near infrared spectrum. In addition, the safety of the nanoformulation was assessed with normal human gingival fibroblasts. Results showed that a significant bacterial killing was measured when biofilms were exposed to an increase of 10°C for a short time of 2 min. Moreover, no cytotoxicity was measured when normal gingival fibroblasts were exposed to the nanoformulation using the bactericidal conditions. The development of the reported formulation can be used as a direct application to treat periodontal diseases or biofilm-produced bacteria that colonize the oral cavity.
📖 Papers frequently viewed together
60 Citations
27 Citations
24 Citations
#1Panchanathan Manivasagan (Pukyong National University)H-Index: 30
#2Subramaniyan Bharathiraja (Pukyong National University)H-Index: 17
Last. Junghwan Oh (Pukyong National University)H-Index: 33
view all 9 authors...
Photothermal therapy (PTT) using biocompatible nanomaterials have recently attracted much attention as a novel candidate technique for cancer therapy. In this work we report the performance of newly synthesized multidentate chitosan oligosaccharide modified gold nanorods (AuNRs-LA-COS) as novel agents for PTT of cancer cells due to their excellent biocompatibility, photothermal stability, and high absorption in the near-infrared (NIR) region. The AuNRs-LA-COS exhibit a strong NIR absorption peak...
18 CitationsSource
#1Yael N. Slavin (UBC: University of British Columbia)H-Index: 4
#2Jason Asnis (UBC: University of British Columbia)H-Index: 2
Last. Horacio Bach (UBC: University of British Columbia)H-Index: 28
view all 4 authors...
As the field of nanomedicine emerges, there is a lag in research surrounding the topic of nanoparticle (NP) toxicity, particularly concerned with mechanisms of action. The continuous emergence of bacterial resistance has challenged the research community to develop novel antibiotic agents. Metal NPs are among the most promising of these because show strong antibacterial activity. This review summarizes and discusses proposed mechanisms of antibacterial action of different metal NPs. These mechan...
588 CitationsSource
#3Tiefeng Jin (Seoul National University Hospital)H-Index: 4
#4Jisu Woo (Seoul National University Hospital)H-Index: 8
Last. Woo Kyung Moon (Seoul National University Hospital)H-Index: 77
view all 6 authors...
// Meihua Zhang 1, 2, 3, * , Hoe Suk Kim 1, * , Tiefeng Jin 1, 4 , Jisu Woo 1 , Yin Ji Piao 1, 2 and Woo Kyung Moon 1, 2 1 Department of Radiology, Seoul National University Hospital, Jongno-gu, Seoul 03080, Korea 2 Department of Biomedical Sciences, Seoul National University College of Medicine, Jongno-gu, Seoul 110-799, Korea 3 Department of Radiology, Yanbian University Hospital, JiLin Province 133000, China 4 Department of Pathology and Cancer Research Center, Yanbian University Medical Coll...
20 CitationsSource
#1Mario Almada (Universidad de Sonora)H-Index: 5
#2B.H. Leal-Martínez (Universidad de Sonora)H-Index: 1
Last. Josué Juárez (Universidad de Sonora)H-Index: 16
view all 8 authors...
Abstract Gold nanorods (GNR) use has been proposed in medical applications because of their intrinsic photothermal properties. However, the presence of CTAB molecules adsorbed onto the surface of GNRs results in a highly cytotoxic GNR system. In this work we replace the CTAB molecules with a thiolated chitosan. Once chitosan coated GNRs (Chi-SH-GNR) were attained, a film of alginate (Alg-Chi-SH-GNR) or polyvinyl alcohol (PVA-Chi-SH-GNR) was deposited onto the surface of Chi-GNR by a layer-by-lay...
34 CitationsSource
#1Meihua Zhang (Yanbian University)H-Index: 4
#2Hoe Suk Kim (New Generation University College)H-Index: 1
Last. Woo Kyung Moon (SNU: Seoul National University)H-Index: 77
view all 4 authors...
Abstract Although triple negative breast cancer (TNBC) is a small percentage of all breast cancers, to date, TNBC is one of the most challenging types of breast cancer for basic and clinic research because TNBC patients display a high risk of relapse, shorter overall survival and limited therapeutic options after completion of conventional chemotherapy compared with patients with other breast cancer subtypes. The epidermal growth factor receptor (EGFR) is a promising target for TNBC treatment. A...
29 CitationsSource
#1Alireza Gharatape (University of Tabriz)H-Index: 3
#2Morteza Milani (University of Tabriz)H-Index: 19
Last. Roya Salehi (University of Tabriz)H-Index: 27
view all 7 authors...
Nowadays the control of multidrug resistant (MDR) and pandrug resistant (PDR) bacteria has been the subject of extensive research. In this study an effective strategy was developed to destroy bacteria using low-level laser photothermal therapy combined with biocompatible surface-modified AuNPs. First, chitosan, poly(N-isopropylacrylamide)-co-(2-dimethylamino ethyl methacrylate) P(NIPAAM-co-DMAEMA) and poly(lactic-co-glycolic acid) (PLGA) modified AuNPs were synthesized and fully characterized. A...
13 CitationsSource
#1Marlos Barbosa-Ribeiro (State University of Campinas)H-Index: 4
#2Adriana de-Jesus-Soares (State University of Campinas)H-Index: 5
Last. Brenda Paula Figueiredo de Almeida Gomes (State University of Campinas)H-Index: 74
view all 6 authors...
Abstract Introduction The aim of this study was to investigate the prevalence of virulence factors and the antimicrobial resistance of Enterococcus faecalis isolates of teeth with failure of the endodontic treatment. Methods Twenty root canal samples were collected from teeth with apical periodontitis. E. faecalis was firstly identified based on phenotypic features and then by 16S ribosomal RNA gene sequencing. The antimicrobial susceptibility was determined by the minimum inhibitory concentrati...
43 CitationsSource
#1Choon Peng Teng (Agency for Science, Technology and Research)H-Index: 15
#2Tielin Zhou (Agency for Science, Technology and Research)H-Index: 1
Last. Ming-Yong Han (Agency for Science, Technology and Research)H-Index: 70
view all 10 authors...
With the rapid evolution of antibiotic resistance in bacteria, antibiotic-resistant bacteria (in particular, multidrug-resistant bacteria) and their biofilms have been becoming more and more difficult to be effectively treated with conventional antibiotics. As such, there is a great demand to develop a nonantibiotic approach in efficiently eliminating such bacteria. Here, multibranched gold nanocrosses with strong near-infrared absorption falling in the biological window, which heat up quickly u...
104 CitationsSource
#1Ruirui Xing (Yanshan University)H-Index: 12
#2Kai Liu (CAS: Chinese Academy of Sciences)H-Index: 27
Last. Xuehai Yan (CAS: Chinese Academy of Sciences)H-Index: 67
view all 9 authors...
An injectable and self-healing collagen-gold hybrid hydrogel is spontaneously formed by electrostatic self-assembly and subsequent biomineralization. It is demonstrated that such collagen-based hydrogels may be used as an injectable material for local delivery of therapeutic agents, showing enhanced antitumor efficacy.
501 CitationsSource
#1Mario Alberto Pérez-Díaz (Universidad Autónoma de San Luis Potosí)H-Index: 5
#2Elizabeth Alvarado-Gomez (Universidad Autónoma de San Luis Potosí)H-Index: 4
Last. Fidel Martinez-Gutierrez (Universidad Autónoma de San Luis Potosí)H-Index: 13
view all 10 authors...
The development of multi-species biofilms in chronic wounds is a serious health problem that primarily generates strong resistance mechanisms to antimicrobial therapy. The use of silver nanoparticles (AgNPs) as a broad-spectrum antimicrobial agent has been studied previously. However, their cytotoxic effects limit its use within the medical area. The purpose of this study was to evaluate the anti-biofilm capacity of chitosan gel formulations loaded with AgNPs, using silver sulfadiazine (SSD) as ...
58 CitationsSource
Cited By6
#1Daqian Gao (Cornell University)
#2Yidan Zhang (Cornell University)
Last. Minglin Ma (Cornell University)
view all 5 authors...
Diabetic wounds often have a slow healing process and become easily infected owing to hyperglycemia in wound beds. Once planktonic bacterial cells develop into biofilms, the diabetic wound becomes more resistant to treatment. Although it remains challenging to accelerate healing in a diabetic wound due to complex pathology, including bacterial infection, high reactive oxygen species, chronic inflammation, and impaired angiogenesis, the development of multifunctional hydrogels is a promising stra...
#1Guijian Guan (TJU: Tianjin University)H-Index: 9
#2Khin Yin Win (Agency for Science, Technology and Research)H-Index: 21
Last. Ming-Yong Han (TJU: Tianjin University)H-Index: 70
view all 5 authors...
With the wide utilization of antibiotics, antibiotic-resistant bacteria have been often developed more frequently to cause potential global catastrophic consequences. Emerging photothermal ablation has been attracting extensive research interest for quick/effective eradication of pathogenic bacteria from contaminated surroundings and infected body. In this field, anisotropic gold nanostructures with tunable size/morphologies have been demonstrated to exhibit their outstanding photothermal perfor...
6 CitationsSource
#2Fahimeh SoleimaniH-Index: 2
Last. Talat BaranH-Index: 37
view all 6 authors...
1 CitationsSource
#1Montserrat Lopez-Carrizales (Universidad Autónoma de San Luis Potosí)H-Index: 2
#2E. Mendoza-Mendoza (Universidad Autónoma de San Luis Potosí)H-Index: 7
Last. Fidel Martinez-Gutierrez (Universidad Autónoma de San Luis Potosí)H-Index: 13
view all 12 authors...
Abstract The purpose of this study was to generate novel chitosan hydrogels (CHs) loaded with silver nanoparticles (AgNPs) and ampicillin (AMP) to prevent early formation of biofilms. AgNPs and CHs were characterized by UV-Vis, DLS, TEM, rheology, FT-IR, Raman, and SEM. The antibiofilm effect of the formulations was investigated against four multidrug-resistant and extensively drug-resistant pathogens using a colony biofilm, a high cell density and gradients model. Also, their hemostatic propert...
3 CitationsSource
#1Ana B. da SilvaH-Index: 2
#2Késsily B. RufatoH-Index: 5
Last. Alessandro F. Martins (CSU: Colorado State University)H-Index: 25
view all 8 authors...
Abstract Conventional strategies (Turkevich's, and modified Turkevich's methods) often synthesize gold nanoparticles (AuNPs). These pathways produce AuNPs using toxic chemistries to reduce Au(III) and stabilize Au(0) atoms upon the AuNP surfaces. To overcome the disadvantages of conventional approaches, chitosan and chitosan-based materials associate with Au(III) to produce composites. Chitosan and derivatives reduce Au(III) and stabilize AuNPs, promoting biocompatibility to the composites, foll...
21 CitationsSource
#1Yosman Dhar (SBU: Stony Brook University)H-Index: 1
#2Yangha Han (SBU: Stony Brook University)H-Index: 1
Abstract Biofilm formation in wounds and implants can have numerous negative effects on a patient's health, including delayed healing and implant removal. Current treatments for biofilms do not completely eradicate microbial colonies or prevent their formation, implying the need for further investigation. Recent research into treatments for wound and implant biofilm infections focus on nanotechnology-based mechanisms for drug delivery, combined therapies, and implant modifications. Ultrasound de...
6 CitationsSource