Histone

Gene

Cell biology

Molecular biology

Biology

Transposable element

DNA methylation

Epigenomics

Computational biology

Genetics

Chromatin

Genome

Transposase

Gene expression

Nature Communications

Many chromatin features play critical roles in regulating gene expression. A complete understanding of gene regulation will require the mapping of specific chromatin features in small samples of cells at high resolution. Here we describe Cleavage Under Targets and Tagmentation (CUT&amp;Tag), an enzyme-tethering strategy that provides efficient high-resolution sequencing libraries for profiling diverse chromatin components. In CUT&amp;Tag, a chromatin protein is bound in situ by a specific antibody, which then tethers a protein A-Tn5 transposase fusion protein. Activation of the transposase efficiently generates fragment libraries with high resolution and exceptionally low background. All steps from live cells to sequencing-ready libraries can be performed in a single tube on the benchtop or a microwell in a high-throughput pipeline, and the entire procedure can be performed in one day. We demonstrate the utility of CUT&amp;Tag by profiling histone modifications, RNA Polymerase II and transcription factors on low cell numbers and single cells.

CUT&amp;Tag for efficient epigenomic profiling of small samples and single cells

CUT&amp;Tag for efficient epigenomic profiling of small samples and single cells

CUT&Tag for efficient epigenomic profiling of small samples and single cells