Anatomy

Force spectroscopy

Cancer

Biology

Optics

Biophysics

Genetics

Cancer cell

Microscopy

Atomic force microscopy

Nanotechnology

Kelvin probe force microscope

Chemistry

Materials science

Ultrastructure

Physics

Journal of Nanobiotechnology

Atomic force microscopy (AFM) allows for nanometer-scale investigation of cells and molecules. Recent advances have enabled its application in cancer research and diagnosis. The physicochemical properties of live cells undergo changes when their physiological conditions are altered. These physicochemical properties can therefore reflect complex physiological processes occurring in cells. When cells are in the process of carcinogenesis and stimulated by external stimuli, their morphology, elasticity, and adhesion properties may change. AFM can perform surface imaging and ultrastructural observation of live cells with atomic resolution under near-physiological conditions, collecting force spectroscopy information which allows for the study of the mechanical properties of cells. For this reason, AFM has potential to be used as a tool for high resolution research into the ultrastructure and mechanical properties of tumor cells. This review describes the working principle, working mode, and technical points of ato...

Application of atomic force microscopy in cancer research