Rapid discrimination and quantification of isomeric flavonoid-O-diglycosides in Citrus paradisi cv. changshanhuyou by online extraction–quadrupole time-of flight tandem mass spectrometry

Rapid differentiation, characterization and quantification of isomers from complex mixtures by direct mass spectrometry (MS) remained an analytical challenge due to their similar or identical MS/MS spectra and matrix interferences. Here, we reported a novel online extraction–quadrupole time-of-flight tandem mass spectrometry (OLE–QTOF-MS/MS) system to rapid, efficient and sensitive analysis of interglycosidic linkage isomers (hesperidin and...