Abstract In this study, effects of icariin (Ica) on were examined in a mouse model of d -galactose (D-gal)-induced ovarian aging. Kunming white mice were divided into three groups: aging group induced with D-gal, experiment group treated with Ica at low (50 mg/kg), middle (100 mg/kg) and high (200 mg/kg) concentrations, and control group with no treatment. Ovarian histomorphology, serum FSH, LH and E2 levels, and reproductive function were compared among the groups. Ovarian expression of Amh, Bax and Bcl-2 was examined by qPCR and western blotting. Our results showed that diameters of secondary and tertiary follicles were significantly reduced in the aging group when compared with control group (P
The network of protein–protein interactions among the BCL-2 protein family plays a critical role in regulating cellular commitment to mitochondrial apoptosis. Anti-apoptotic BCL-2 proteins are considered promising targets for drug discovery and exciting clinical progress has stimulated intense investigations in the broader family. Here, we discuss recent developments in small molecules targeting anti-apoptotic proteins and alternative approaches to targeting BCL-2 family interactions. These stud...
#1Wenwen Ma(HUST: Huazhong University of Science and Technology)H-Index: 4
#2Jing Xiao(HUST: Huazhong University of Science and Technology)H-Index: 3
Last. Mingmin Zhang(HUST: Huazhong University of Science and Technology)H-Index: 9
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The effect and underlying mechanism of Bu-Shen-An-Tai recipe on ovarian apoptosis in mice with controlled ovarian hyperstimulation (COH) implantation dysfunction were studied. The COH implantation dysfunction model in mice was established by intraperitoneal injection of 7.5 IU pregnant mare’s serum gonadotrophin (PMSG), followed by 7.5 IU human chorionic gonadotrophin (HCG) 48 h later. Then the female mice were mated with male at a ratio of 2:1 in the same cage at 6:00 p.m. The female mice from ...
This study aimed to investigate the possible molecular mechanisms of active ingredients of Epimedii Folium (EF) and Ligustri Lucidi Fructus (LLF) combined with Budesonide (Bun) in asthmatic rats. Rats were divided into 5 groups, including normal group, asthma model group, Bun group, group of active ingredients of EL and LLF (EL), and group of coadministration of Bun with EL (Bun&EL). The asthmatic model was prepared by ovalbumin sensitizing and challenging. Lymphocyte apoptosis, GR protein and b...
Last. Saad Amer(University of Nottingham)H-Index: 19
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Background Antipsychotics (APs) are widely prescribed drugs, which are well known to cause reproductive adverse effects through mechanisms yet to be determined. The purpose of this study was to investigate the effect of antipsychotics on mitochondrial bioenergetics of rat ovarian theca cells as a possible mechanism of reproductive toxicity. Methods Isolated rat theca interstitial cells (TICs) were treated with two typical (chlorpromazine [CPZ] and haloperidol [HAL]) and two atypical APs (risperi...
#1Åsa Magnusson(Sahlgrenska University Hospital)H-Index: 3
#2Lars Nilsson(Sahlgrenska University Hospital)H-Index: 19
Last. Christina Bergh(Sahlgrenska University Hospital)H-Index: 54
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Abstract Does the addition of anti-Mullerian hormone (AMH) to a conventional dosage regimen, including age, antral follicle count (AFC) and BMI, improve the rate of targeted ovarian response, defined as 5-12 oocytes after IVF? The addition of AMH did not alter the rate of targeted ovarian response, 5-12 oocytes, or decreased the rate of ovarian hyperstimulation syndrome (OHSS) or cancelled cycles due to poor ovarian response. Controlled ovarian hyperstimulation (COH) in connection with IVF is so...
#1Lianli He(CQMU: Chongqing Medical University)H-Index: 1
#2Li Ling(CQMU: Chongqing Medical University)H-Index: 5
Last. Zhengai Xiong(CQMU: Chongqing Medical University)H-Index: 6
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This study aims to investigate the effect as well as mechanism of ginsenoside Rg1 (Rg1) on premature ovarian failure (POF) induced by d-galactose (d-gal) in mice. C57BL/6 female mice were divided into four groups randomly, which were the saline group, the d-gal group, the d-gal + Rg1 group, and the Rg1 group. Body weight was recorded. Overall ovarian function including estrous cycles, sex hormone secretion, ovarian follicle development, and ovarian morphology was analyzed by H&E staining and ELI...
Last. Elizabeth Olufunke Oke(LAUTECH: Ladoke Akintola University of Technology)H-Index: 1
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Background: Assessment of ovarian reserve is one of the steps in the management of infertile couples. Follicle Stimulating hormone (FSH) and anti-Mullerian hormone (AMH) are commonly used ovarian reserve markers in Africa. However, there is paucity of age-specific reference values for FSH and AMH among the African population.Objective: This study aimed at conducting a pilot study for generation of age-specific nomograms for FSH and AMH among fertile women in Ile-Ife, Nigeria.Materials and Method...
Last. Leon J. Spicer(OSU: Oklahoma State University–Stillwater)H-Index: 52
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Abstract Fibroblast growth factor 9 (FGF9) has been suggested to act as an antidifferentiation factor in cattle by reducing steroidogenesis and increasing cell proliferation in granulosa (GC) and theca (TC) cells. The objective of this study was to characterize FGF9 mRNA abundance in GC and TC during development of dominant follicles in dairy cattle. Estrous cycles of nonlactating dairy cattle were synchronized, and ovaries were collected on either d 3 to 4 (n=8) or 5 to 6 (n=8) postovulation fo...
#1Shuhang Liang(HMU: Harbin Medical University)H-Index: 1
#2Kuo Sun(HMU: Harbin Medical University)H-Index: 1
Last. YongHui Wu(HMU: Harbin Medical University)H-Index: 1
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Abstract Zinc oxide nanoparticles (ZnO NPs) are widely used in a variety of products used in daily life. However, their impact on human health has not been completely elucidated. This study was designed to investigate the cytotoxicity associated with ZnO NPs, the role of dissolution in the toxicity of ZnO NPs, the molecular mechanisms and mode of cell death induced by ZnO NPs in human aortic endothelial cells (HAECs), and the protective effects of the antioxidant alpha-lipoic acid (LA). ZnO NPs ...
#1Suzhen Yuan(HUST: Huazhong University of Science and Technology)H-Index: 5
#2Jingyi Wen(HUST: Huazhong University of Science and Technology)H-Index: 2
Last. Shixuan Wang(HUST: Huazhong University of Science and Technology)H-Index: 31
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ABSTRACTFollicular atresia is the main process responsible for the loss of follicles and oocytes from the ovary, and it is the root cause of ovarian aging. Apoptosis of granulosa cells (GCs) is the cellular mechanism responsible for follicular atresia in mammals. Recent advances have highlighted fundamental roles for EGR1 in age-related diseases via the induction of apoptosis. In the present study, we found that the expression of EGR1 was significantly increased in aged mouse ovaries compared wi...
#1Lingdi Wang(Guangzhou University of Chinese Medicine)
#2Jian Liu(Guangzhou University of Chinese Medicine)H-Index: 2
Last. Hongyan Yang(Guangzhou University of Chinese Medicine)H-Index: 6
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Premature ovarian insufficiency (POI) is a common female endocrine disease that is closely linked to ovarian function. Danggui Buxue Tang (DBT) is a classic prescription of traditional Chinese medicine that is helpful for rescuing ovarian function. However, the mechanism by which DBT rescues ovarian function remains unclear. This study explored the molecular mechanism of DBT with respect to apoptosis and related signals in ovarian cells. The quality control of DBT was performed by HPLC. After DB...
#1Min-You Qi(Zhejiang University of Technology)H-Index: 4
#2Ying-Hao He(Zhejiang University of Technology)H-Index: 1
Last. Jia-Qi Hao(Zhejiang University of Technology)
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Diabetic nephropathy (DN) is one of the complex and severe complications of diabetes mellitus (DM). Icariin (ICA) is a flavonoid extracted from the leaves and stems of Herba epimedii with a wide range of pharmacological effects, such as anti-osteoporosis, anti-fibrosis, anti-aging, anti-inflammation and antioxidation. The purpose of our study was to explore the renal protective effect of ICA on DN in mice and its possible mechanisms. ICR mice were exposed to STZ-induced DN. The kidney organ coef...
The ovarian system is comprised of vital organs in females as they are of great significance for maintaining reproductive potential and endocrine stability. Female ovarian aging occurs far earlier than the aging of other organs, and is a pacemaker of female body aging. This drives the aging of multiple organs and leads to several aging-related disorders. Although complex pathogenesis undoubtedly contributes to ovarian aging, increasing attention has been paid to the extensive influence of oxidat...
#1Weiping Liu(Guangzhou University of Chinese Medicine)
#2Qi Chen(Guangzhou University of Chinese Medicine)
Last. Shao-Hu Zhou(Guangzhou University of Chinese Medicine)
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Abstract Ethnopharmacological relevance Zihuai recipe (ZHR), a Chinese herbal prescription, is widely used for the clinical treatment of Diminished ovarian reserve (DOR) infertility. However, little is known regarding its underlying mechanisms of DOR treatment. Aim of the study This study aimed to investigate the beneficial effects of ZHR on the treatment of DOR and to reveal the underlying mechanisms. Materials and methods Sixty female 8-week-old Sprague–Dawley rats were randomly divided into t...
Losing of ovarian functions prior to natural menopause age causes female infertility and early menopause. Premature ovarian insufficiency (POI) is defined as the loss of ovarian activity before 40 years of age. Known genetic causes account for 25%-30% of POI cases, demonstrating the high genetic heterogeneity of POI and the necessity for further genetic explorations. Here we conducted genetic analyses using whole-exome sequencing in a Chinese non-syndromic POI family with the affected mother and...
#1Changqing Xu(Hangzhou Normal University)H-Index: 15
#2Xuqing Huang(Hangzhou Normal University)H-Index: 2
Last. Yuyue Jiang(Hangzhou Normal University)H-Index: 1
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Icariin (ICA) has been used as a promising anti‑aging drug; however, its underlying molecular mechanism is yet to be elucidated. The present study aimed to determine the anti‑aging molecular mechanisms of ICA. D‑galactose (D‑gal) was used to generate a cell aging model. IMR‑90 human lung fibroblasts were pretreated with different concentrations of ICA (1, 2, 4, 8 and 16 µmol/l) for 6 h and subsequently incubated with D‑gal (200 mmol/l) at 37˚C for 72 h. Senescence of IMR‑90 cells was assessed by...
Purpose. To investigate whether icariin (ICA), a well-known medicine extracted from the stem and leaf of Epimedium brevicornum Maxim, had analgesic effect on lower back pain (LBP) in rats. Methods. In a puncture-induced LBP rat model, the severity of LBP was quantified using the paw/foot withdrawal threshold method after intragastric administration of ICA at a dosage of 50 mg/kg/d or 100 mg/kg/d. The pain-related peptides of substance P (SP) and calcitonin gene-related peptide (CGRP) were also m...
Last. Zhongxin Zhou(HAU: Huazhong Agricultural University)H-Index: 1
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Osteoporosis is a common chronic disease in the elderly population and some domestic animals. Caged layer osteoporosis (CLO) is a common bone metabolism disease which was recently recommended as an ideal animal model for osteoporosis. This study aimed to investigate the therapeutic effect and mechanism of dietary icariin (ICA), the main bioactive components of Chinese herb Epimedium, on low bone mineral density (BMD) in older caged laying hens. A total of 216 Lohmann pink-shell laying hens of 54...
Last. Shengyan Xi(Ha Tai: Xiamen University)H-Index: 6
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Background: Chronic stress has been known to impair the female reproductive function, but the mechanism remains to be further investigated. Chaiyu-Dixian Formula (CYDXF) has been reported to regulate human endocrine disorders clinically. However, whether this formula can affect chronic stress-induced ovarian follicular development is not clear. Aim of the study: To examine effects of CYDXF on follicular development and explore possible mech anisms in a chronic unpredictable mild stress (CUMS) mo...
Last. Xiaogang Du(Sichuan Agricultural University)H-Index: 11
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BACKGROUND: Oyster polypeptides have various biofunctions, such as anti-cancer and anti-oxidative stress, but whether it has the protective effects to primary ovarian failure (POF) remains poorly understand. To address this issue, daily gavage of oyster polypeptides was performed to investigate their protective effect, basing on d-galactose-induced POF model in C57BL/6 female mice. RESULTS: Oyster polypeptides restored the irregular estrous cycles and the abnormal serum follicle stimulating horm...