A rapid ATR-FTIR spectroscopic method for classification of gelatin gummy candies in relation to the gelatin source.

Published on Mar 30, 2019in Food Chemistry6.306
· DOI :10.1016/J.FOODCHEM.2018.10.125
Nur Cebi7
Estimated H-index: 7
(YTU: Yıldız Technical University),
Canan Ekinci Dogan6
Estimated H-index: 6
(Scientific and Technological Research Council of Turkey)
+ 3 AuthorsOsman Sagdic44
Estimated H-index: 44
(YTU: Yıldız Technical University)
Abstract Gelatin is widely used in gummy candies because of its unique functional properties. Generally, porcine and bovine gelatins are used in the food industry. FTIR-ATR combined with chemometrics analysis such as hierarchical cluster analysis (HCA) (OPUS Version 7.2 software), principal component analysis (PCA) (OPUS Version 7.2 software) and partial least squares-discriminant analysis (PLS-DA) (Matlab R2017b) were used for classification and discrimination of gelatin gummy candies related to their gelatin source. The spectral region between 1734 and 1528 cm−1 was selected for chemometric analysis. The potential of FTIR spectroscopy for determination of bovine and porcine source in gummy candies was examined and validated by a real-time polymerase chain reaction (PCR) method. Twenty commercial samples were tested by developed ATR-FTIR methodology and RT-PCR technique, mutually confirming and supporting results were obtained. Gummy candies were classified and discriminated in relation to the bovine or porcine source of gelatin with 100% success without any sample preparation using FTIR-ATR technique.
📖 Papers frequently viewed together
1 Author (Norakasha Rusli)
118 Citations
6 Authors (Yahong Han, ..., Xian Liu)
1 Citations
#1Behrooz Jannat (FDA: Food and Drug Administration)H-Index: 17
#2Kazem GhorbaniH-Index: 2
Last. Mohsen SadeghiH-Index: 1
view all 10 authors...
Abstract Authentication of gelatin, an ingredient in food and drug products, is of interest with respect to some regulations and religion rules. Determination of the gelatin origin is particularly challenging because of the similarity in amino acid sequences of collagen types in different species. In this study, Polymerase Chain Reaction (PCR) technique using species-specific primers and also chemometrics analysis of mass spectral data were investigated to differentiate bovine, porcine and fish s...
12 CitationsSource
#1Nur Cebi (YTU: Yıldız Technical University)H-Index: 7
#2Mustafa Yilmaz (YTU: Yıldız Technical University)H-Index: 52
Last. Osman Sagdic (YTU: Yıldız Technical University)H-Index: 44
view all 3 authors...
Abstract Sibutramine may be illicitly included in herbal slimming foods and supplements marketed as “100% natural” to enhance weight loss. Considering public health and legal regulations, there is an urgent need for effective, rapid and reliable techniques to detect sibutramine in dietetic herbal foods, teas and dietary supplements. This research comprehensively explored, for the first time, detection of sibutramine in green tea, green coffee and mixed herbal tea using ATR-FTIR spectroscopic tec...
40 CitationsSource
#1Nur Cebi (YTU: Yıldız Technical University)H-Index: 7
#2Canan Ekinci Dogan (Scientific and Technological Research Council of Turkey)H-Index: 6
Last. Osman Sagdic (YTU: Yıldız Technical University)H-Index: 44
view all 5 authors...
Abstract l -Cysteine is deliberately added to various flour types since l -Cysteine has enabled favorable baking conditions such as low viscosity, increased elasticity and rise during baking. In Turkey, usage of l -Cysteine as a food additive isn’t allowed in wheat flour according to the Turkish Food Codex Regulation on food additives. There is an urgent need for effective methods to detect l -Cysteine in wheat flour. In this study, for the first time, a new, rapid, effective, non-destructive an...
29 CitationsSource
#1Hatice Saadiye Eryılmaz (ITU: Istanbul Technical University)H-Index: 1
#2Beyza Şükran Işık (ITU: Istanbul Technical University)H-Index: 1
Last. Dilara Nilüfer Erdil (ITU: Istanbul Technical University)H-Index: 1
view all 6 authors...
Gelatin origin determination has been a crucial issue with respect to religion and health concerns. It is necessary to analyze the origin of gelatin with reliable methods to ensure not only consumer choices but also safety and legal requirements such as labeling. There are many analytical methods developed for detection and/or quantification of gelatin from different sources including bovine, porcine and piscine. These analytical methods can be divided into physicochemical, chromatographic, immu...
5 CitationsSource
#1Aziz HirriH-Index: 1
#2Mahfould BassbasiH-Index: 1
Last. Abdelkhalek OussamaH-Index: 13
view all 5 authors...
Fourier transform infrared (FTIR) coupled to chemometrics was shown to be a useful method to classify and predict the quality of four commercial grade virgin olive oils (VOO). FTIR and physicochemical data were collected using a set of 70 samples representing extra virgin (EV), virgin (V), ordinary virgin (OV), and lampante (L) commercial grade olive oils collected in Beni Mellal region (central Morocco). Two partial least squares discriminant analysis (PLS-DA) models using physicochemical data ...
40 CitationsSource
#1Nur Cebi (YTU: Yıldız Technical University)H-Index: 7
#2M. Zeki Durak (YTU: Yıldız Technical University)H-Index: 11
Last. Muhammet Arici (YTU: Yıldız Technical University)H-Index: 19
view all 5 authors...
Abstract The objective of this research was to develop a rapid spectroscopic technique as an alternative method for the differentiation and authentication of gelatin sources in food products by using attenuated total reflectance Fourier transform infrared (ATR–FTIR) spectra combined with chemometrics. Clear discrimination and classification of all the studied gelatin sources (bovine, porcine, and fish) were achieved by hierarchical cluster and principle component analysis (PCA). Amide-I (1700–16...
86 CitationsSource
#1Piotr S. Gromski (University of Manchester)H-Index: 12
#2Howbeer Muhamadali (University of Manchester)H-Index: 16
Last. Royston Goodacre (University of Manchester)H-Index: 100
view all 7 authors...
The predominance of partial least squares-discriminant analysis (PLS-DA) used to analyze metabolomics datasets (indeed, it is the most well-known tool to perform classification and regression in metabolomics), can be said to have led to the point that not all researchers are fully aware of alternative multivariate classification algorithms. This may in part be due to the widespread availability of PLS-DA in most of the well-known statistical software packages, where its implementation is very ea...
368 CitationsSource
#1Seher Gok (METU: Middle East Technical University)H-Index: 4
#2Mete Severcan (METU: Middle East Technical University)H-Index: 13
Last. Feride Severcan (METU: Middle East Technical University)H-Index: 35
view all 5 authors...
Abstract Botanical origin of the nectar predominantly affects the chemical composition of honey. Analytical techniques used for reliable honey authentication are mostly time consuming and expensive. Additionally, they cannot provide 100% efficiency in accurate authentication. Therefore, alternatives for the determination of floral origin of honey need to be developed. This study aims to discriminate characteristic Anatolian honey samples from different botanical origins based on the differences ...
90 CitationsSource
#1Ofélia Anjos (IPN: Instituto Politécnico Nacional)H-Index: 18
#2Maria Gabriela Nogueira Campos (UC: University of Coimbra)H-Index: 24
Last. P. AntunesH-Index: 2
view all 4 authors...
Abstract A Fourier transform infrared spectroscopic method with attenuated total reflectance (FTIR-ATR) and partial least squares (PLS) regression model for the prediction of sugar content in honey samples was calculated. Standards of trehalose, glucose, fructose, sucrose, melezitose, turanose and maltose were used to identify and quantify the individual sugar components in 63 honey samples by HPAEC-IPAD. Fructose and glucose are the highest sugars in honey with an average value of 36% and 26%, ...
138 CitationsSource
#1Athina I. Ropodi (AUA: Agricultural University of Athens)H-Index: 5
#2Dimitrios Pavlidis (AUA: Agricultural University of Athens)H-Index: 4
Last. George Nychas (AUA: Agricultural University of Athens)H-Index: 5
view all 5 authors...
Abstract The aim of this study was to investigate the potential of multispectral imaging supported by multivariate data analysis for the detection of minced beef fraudulently substituted with pork and vice versa. Multispectral images in 18 different wavelengths of 220 meat samples in total from four independent experiments (55 samples per experiment) were acquired for this work. The appropriate amount of beef and pork-minced meat was mixed in order to achieve nine different proportions of adulte...
81 CitationsSource
Cited By18
#1Alaitz Etxabide (University of Auckland)H-Index: 16
#2Paul A. Kilmartin (University of Auckland)H-Index: 46
Last. Rafea NaffaH-Index: 7
view all 6 authors...
Abstract The Maillard Reaction (MR), a condensation reaction between carbonyl-containing and free amino-containing compounds, has attracted the interest of scientists within the food industry, nutrition & health, and materials science. However, due to the complexity of the reaction much remains to be clarified regarding the nature of the reaction products and their effect on the properties of protein/saccharide-containing products. In this work, gelatin-based films with various saccharides (gluc...
#1Lefan Li (Ministry of Education)H-Index: 2
#2Qiao Wang (Ministry of Education)H-Index: 4
Last. Guoxing Sun (Ministry of Education)H-Index: 20
view all 6 authors...
Abstract Most studies on enhanced polymer gels look at modifying the interaction between the polymer matrix and fillers. Here, a new strategy is proposed for the fabrication of a new kind of polyacrylamide/Ca(OH)2 nanocomposite organogel with tunable mechanical properties and temperature tolerance. By changing the solvent component, the properties of an organogel can be modulated without adding further reinforcing agent. Two commonly used organic solvents, glycerin (GL) and ethylene glycol (EG),...
#1Rui Lang (Qilu University of Technology)
#2Mao Yang (Qilu University of Technology)H-Index: 2
Last. Xugang Dang (Shaanxi University of Science and Technology)
view all 5 authors...
Abstract A potential nanocomposite scaffold based on waterborne polyurethane grafted collagen polypeptide/hydroxyapatite (WPU-g-CP/HAp) was investigated in this research. First, a non-solvated WPU-g- CP copolymer emulsion was synthesized via water phase cross-linking copolymerization between waterborne polyurethane (WPU) prepolymer and KH550 modified collagen polypeptide (CP). Then, the WPU-g-CP/HAp was fabricated employing bionic precipitation method of HAp nanocrystals, followed by freeze-dryi...
#1Wenguang Wei (CAU: China Agricultural University)
#2Qi Luo (CAU: China Agricultural University)H-Index: 2
Last. Min Wu (CAU: China Agricultural University)H-Index: 3
view all 8 authors...
Pinggu Peach Orchard is one of the largest orchards in Beijing, China, providing consumers with high-quality peaches. However, a large number of discarded peach branch waste was produced along with the peach production every year, which can cause environmental pollution and have potential safety hazards since they can only be burned or stacked on the roadside or in the field. Therefore, it is important and urgent to develop recycling techniques of discarded peach branches. In this study, peach b...
#1Yan-Li Lü (Heilongjiang University of Chinese Medicine)
#2Jun Liang (Heilongjiang University of Chinese Medicine)H-Index: 12
Last. Yong-Gang Xia (Heilongjiang University of Chinese Medicine)H-Index: 20
view all 5 authors...
Abstract In this study, a novel two-dimensional correlation infrared spectroscopy (2DCOS-IR) is presented to rapidly characterize and discriminate polysaccharides in Panax ginseng (PGP), P. notoginseng (PNP), and P. quinquefolius (PQP) using attenuated total reflection Fourier transform infrared spectroscopy–based on single-characteristic temperature as the external disturbance (2D-sATR-FTIR). Compared with two existing 2DCOS-IR methods based on gradient heating pathways using KBr pellet (100 mi...
#1Iago José Santos da Silva (UFRPE: Universidade Federal Rural de Pernambuco)H-Index: 2
#2Matheus Lima de Mello (UFSCar: Federal University of São Carlos)H-Index: 1
Last. Maria José da Silva (UFRPE: Universidade Federal Rural de Pernambuco)H-Index: 8
view all 6 authors...
Recently, consumption of collagen-based foodstuffs has grown significantly, especially collagen hydrolysate, used as a dietary supplement. Information available on the content of essential and toxic elements in these products, however, is incipient. This study provides a novel assessment of the mineral profile (Al, As, Ca, Cd, Co, Cr, Fe, K, Mg, Mn, Na, P, S, V, and Zn) of collagen hydrolysate and gelatin by inductively coupled plasma optical emission spectrometry (ICP OES) and mass spectrometry...
#1Mediha Esra Altuntop Yayla (Scientific and Technological Research Council of Turkey)H-Index: 1
#2Canan Ekinci Dogan (Scientific and Technological Research Council of Turkey)H-Index: 6
Porcine adulteration has become a major concern amongst communities including Muslims and Jews. Reliable detection of the presence of porcine DNA in gelatin and highly processed food samples is essential for Halal and kosher food control. In this study, a forensic approach involving DNA isolation and real-time polymerase chain reaction (PCR) was performed to detect the presence of porcine DNA in commercial gelatin and processed foods containing porcine by-products. The method was compared with c...
#1Bing Yan (Cornell University)H-Index: 5
#1Bing Yan (Cornell University)H-Index: 1
Last. Alireza Abbaspourrad (Cornell University)H-Index: 30
view all 9 authors...
Abstract Vitamin gummies have gained popularity in recent years due to ease of swallowing, appealing appearance, and tasty flavors. Water-soluble vitamins such as vitamin C are susceptible to degradation when exposed to oxygen, moisture, light, heat, and change in pH during manufacturing and shelf life. Therefore, it is essential to improve vitamin stability and delay the degradation process. In this study, microencapsulation of vitamin C inside casein gel followed by spray drying produced vitam...
3 CitationsSource
#1Nur Cebi (YTU: Yıldız Technical University)H-Index: 7
#2Muhammet Arici (YTU: Yıldız Technical University)H-Index: 19
Last. Osman Sagdic (YTU: Yıldız Technical University)H-Index: 44
view all 3 authors...
Abstract There is a necessity for rapid, robust, easy, accurate and cost-effective methodologies for the quality control of essential oils from medicinal and aromatic plants. Rosa damascena essential oil is a high-value natural product with its unique quality properties and economic importance. This research evaluated the capability of Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and gas chromatography-mass spectrometry (GC-MS) techniques combined with chemometrics for dete...
#1Nesrin Ecem Bayram (Bayburt University)H-Index: 3
#2Nur Cebi (YTU: Yıldız Technical University)H-Index: 7
Last. Aslı Özkök (Hacettepe University)H-Index: 3
view all 8 authors...
Royal jelly is one of the most remarkable commercial bee products used in cosmetics, medicine, and as a dietary supplement. This study aimed to determine some quality parameters (amino acid, multi-...
1 CitationsSource