Original paper
Evaluation of 244,000 synthetic sequences reveals design principles to optimize translation in Escherichia coli
Abstract
Large and systematic mRNA library design disentangles the complex sequence determinants of translation efficiency in bacteria. Comparative analyses of natural and mutated sequences have been used to probe mechanisms of gene expression, but small sample sizes may produce biased outcomes. We applied an unbiased design-of-experiments approach to disentangle factors suspected to affect translation efficiency in E. coli. We precisely designed 244,000...
Paper Details
Title
Evaluation of 244,000 synthetic sequences reveals design principles to optimize translation in Escherichia coli
Published Date
Sep 24, 2018
Journal
Volume
36
Issue
10
Pages
1005 - 1015