Electrochemiluminescent Imaging for Multi-immunoassay Sensitized by Dual DNA Amplification of Polymer Dot Signal

A true-color electrochemiluminescent (ECL) imaging strategy was designed for a multi-immunoassay of proteins by coupling highly efficient polymer dots (Pdots) with dual DNA amplification. The Pdots were prepared by nanoprecipitation of poly[(9,9-dioctylfuorenyl-2,7-diyl)-alt-co-(1,4-benzo-{2,1′,3}-thiadiazole)] in the presence of poly(styrene-co-maleic anhydride) and functionalized with DNA1 that hybridized with black hole quencher-labeled DNA2...