Multiparameter Affinity Microchip for Early Sepsis Diagnosis Based on CD64 and CD69 Expression and Cell Capture

Sepsis is a leading cause of death worldwide. In this work, a multiparameter affinity microchip was developed for faster sepsis diagnosis, which can reduce the mortality caused by late validation. The separation device captured cells expressing CD25, CD64, and CD69 into discrete antibody regions. The performance of multiparameter cell separation microchips was compared with flow cytometry analysis and validated with samples of septic patients (...