Convenient method for better preservation of fine structures of cultured macrophages and engulfed yeast cells by freeze-substitution fixation
Abstract
Rapid freeze-freeze substitution after glutaraldehyde fixation (CF-FS method) obtained the natural and fine structures of macrophages and engulfed yeast cells. Culturing macrophages on single hole molybdenum grids placed in culture dishes made possible the rapid freezing of cells by the 'open sandwich method'. This method may be convenient when rapid-freezing cannot be performed immediately, or when a rapid-freezing device is not available in...
Paper Details
Title
Convenient method for better preservation of fine structures of cultured macrophages and engulfed yeast cells by freeze-substitution fixation
Published Date
Feb 18, 2017
Volume
66
Issue
3
Pages
209 - 211
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