Glutaminase free l-asparaginase from Vibrio cholerae: Heterologous expression, purification and biochemical characterization

l-asparaginase is used as a pivotal agent in treatment of lympho proliferative disorders and holds an excessive demand for food processing aids. Most of the l-asparaginases possess glutaminase activity which might lead to side effects during the treatment. The search for new therapeutic enzymes is of great interest in both medical and food applications. In this study, Vibrio cholerae l-asparaginase was recombinantly overexpressed in E. coli,...