Concurrent isotope‐assisted metabolic flux analysis and transcriptome profiling reveal responses of poplar cells to altered nitrogen and carbon supply

Summary Reduced nitrogen is indispensable to plants. However, its limited availability in soil combined with the energetic and environmental impacts of nitrogen fertilizers motivates research into molecular mechanisms toward improving plant nitrogen use efficiency (NUE). We performed a systems‐level investigation of this problem by employing multiple ‘omics methodologies on cell suspensions of hybrid poplar ( Populus tremula × Populus alba )....