Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis

Volume: 8, Issue: 1
Published: Nov 10, 2017
Abstract
The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe "semisynthetic" pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and...
Paper Details
Title
Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
Published Date
Nov 10, 2017
Volume
8
Issue
1
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