Interpretive Approaches to Optimize Serially-Coupled Columns in Reversed-Phase Liquid Chromatography

The serial coupling of chromatographic columns of controlled lengths containing different stationary phases multiplies the available selectivity in reversed-phase liquid chromatography, which expands the probabilities of success in the separation of complex samples. However, the full exploitation of the separation capability of coupled columns requires a reliable predictor system of solute retention, peak profile and resolution, for both...