T. Alvarez-Segura
University of Valencia
Distribution (mathematics)CouplingAlgorithmAnalytical chemistryAccountingWork (thermodynamics)Column (database)Two-dimensional chromatographySubdivisionArtificial intelligenceVan Deemter equationVolumetric flow rateSet (abstract data type)Dispersion (optics)Pattern recognitionHigh-performance liquid chromatographyChemistryMaterials scienceLine (geometry)Scale (descriptive set theory)AcetonitrileTandemConstant (mathematics)MethanolMeasure (data warehouse)SolventColumn (typography)Primary amino acidsIsoindolesChromatography liquidStationary phasePhase compositionSeparation problemMedicinal herbsIsocratic elutionArea fractionDirect evaluationGreen teaMass spectrometricDead volumeGradient elutionComparison studyCyano columnLinear gradientKey featuresTheoretical plateLevel of detailChromatographyPhase (matter)Reversed-phase chromatographyExtraction (chemistry)Amino acidExperimental dataChromatography columnFunction (mathematics)Constant linear velocityElutionFlow (psychology)Resolution (mass spectrometry)
12Publications
5H-index
88Citations
Publications 12
Newest
#1T. Alvarez-Segura (University of Valencia)H-Index: 5
#2Sergio López-Ureña (University of Valencia)H-Index: 5
Last. M.C. García-Alvarez-Coque (University of Valencia)H-Index: 31
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Abstract Multi-linear gradients are a convenient solution to get separation of complex samples by modulating carefully the gradient slope, in order to accomplish the local selectivity needs for each particular solute cluster. These gradients can be designed by trial-and-error according to the chromatographer experience, but this strategy becomes quickly inappropriate for complex separations. More evolved solutions imply the sequential construction of multi-segmented gradients. However, this stra...
2 CitationsSource
#1T. Alvarez-Segura (University of Valencia)H-Index: 5
#2E. Cabo-Calvet (University of Valencia)H-Index: 2
Last. M.C. García-Alvarez-Coque (University of Valencia)H-Index: 31
view all 4 authors...
Abstract Performance of chromatographic columns is of major importance in the development of more efficient separation methods. So far, a common practice is to study the column behavior in isocratic elution by modifying the flow rate and fitting the theoretical plate height values versus the mobile phase linear velocity, according to the Van Deemter equation. In this work, an approach is presented to extend the measurement of efficiency to linear gradient elution, where the mean retention factor...
4 CitationsSource
#1T. Alvarez-Segura (University of Valencia)H-Index: 5
#2Jose Ramon Torres-Lapasio (University of Valencia)H-Index: 30
Last. María Celia García-Alvarez-Coque (University of Valencia)H-Index: 21
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The most efficient optimization methodologies in liquid chromatography are based on the modeling and prediction of the chromatographic behavior for each compound in the sample. However, when the column suffers some ageing after the modeling process, predictions may differ significantly from the actual separation. Repeating the modeling is especially troublesome when several columns are involved, as is the case of coupled columns. We propose a shortcut to correct the time and peak profiles in the...
Source
#1T. Alvarez-SeguraH-Index: 5
Last. Juan José Baeza-BaezaH-Index: 19
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3 CitationsSource
#1J.A. Navarro-Huerta (University of Valencia)H-Index: 6
#2T. Alvarez-Segura (University of Valencia)H-Index: 5
Last. M.C. García-Alvarez-Coque (University of Valencia)H-Index: 31
view all 4 authors...
The search for best conditions in liquid chromatography is routinely carried out with information provided by chemical standards. However, sometimes there are samples with insufficient knowledge about their chemical composition. In other cases, identities of the components are known, but there are no standards available, and in other cases the identities of peaks in chromatograms taken under different conditions are ambiguous. Most resolution criteria used to measure separation performance canno...
4 CitationsSource
#1T. Alvarez-SeguraH-Index: 5
Last. M. Celia García-Alvarez-Coque (University of Valencia)H-Index: 4
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Single conventional columns in reversed-phase LC are insufficient for analysing the isoindoles of primary amino acids, due to their limited functionality. An interesting possibility for increasing the separation power is the combination of several columns of different nature, where the length is modified by coupling small segments. This approach may require a considerable investment to have multiple lengths for each stationary phase. However, the combination of only two columns of fixed length c...
8 CitationsSource
#1T. Alvarez-Segura (University of Valencia)H-Index: 5
#2Jose Ramon Torres-Lapasio (University of Valencia)H-Index: 30
Last. M.C. García-Alvarez-Coque (University of Valencia)H-Index: 31
view all 4 authors...
Abstract Liquid chromatography with single columns often does not succeed in the analysis of complex samples, in terms of resolution and analysis time. A relatively simple solution to enhance chromatographic resolution is the modulation of the stationary phase through the serial coupling of columns. This can be implemented with any type of column using compatible elution conditions and conventional instruments. This review describes the key features of column coupling and published procedures, w...
40 CitationsSource
#1T. Alvarez-SeguraH-Index: 5
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2 CitationsSource
#1T. Alvarez-Segura (University of Valencia)H-Index: 5
#2E. Cabo-Calvet (University of Valencia)H-Index: 2
Last. M.C. García-Alvarez-Coque (University of Valencia)H-Index: 31
view all 4 authors...
A new approach is reported for high-performance liquid chromatography to measure the level of information in fingerprints. For this purpose, the concept of peak prominence, which is the protruding part of each visible peak with regard to the valleys that delimit it, was used. The peaks in the fingerprints are ranked according to the areas of the peak prominences, and a threshold is established to discriminate between the significant peaks and those that are irreproducible. The approach was appli...
5 CitationsSource
#1T. Alvarez-SeguraH-Index: 5
Last. J.R. Torres-LapasióH-Index: 4
view all 4 authors...
1 Citations