Identification of Circulating miRNAs Differentially Regulated by Opioid Treatment

Published on Sep 16, 2017in International Journal of Molecular Sciences5.924
· DOI :10.3390/IJMS18091991
Kaoru Toyama4
Estimated H-index: 4
(Daiichi Sankyo),
Naoki Kiyosawa17
Estimated H-index: 17
(Daiichi Sankyo)
+ 1 AuthorsHitoshi Ishizuka10
Estimated H-index: 10
(Daiichi Sankyo)
Sources
Abstract
Emerging evidence demonstrates functional contributions of microRNAs (miRNAs) to μ-opioid receptor (MOR) signaling, but the information so far has been mostly limited to their intracellular regulatory mechanisms. The present study aimed to investigate changes in plasma miRNA profiles elicited by opioid treatment in blood samples collected from clinical studies. Healthy male subjects were orally administered with hydromorphone or oxycodone and blood samples were collected at a specified time after the drug treatment. A total of 179 plasma miRNAs were measured using multiplex qRT-PCR. Nine and seventeen miRNAs were commonly upregulated (let-7a-5p, miR-423-3p, miR-199a-3p, miR-146a-5p, miR-23b-3p, miR-24-3p, miR-221-3p, miR-223-3p, and miR-146b-5p) and downregulated (miR-144-3p, miR-215, miR-363-3p, etc.), respectively, following opioid treatment. The MOR signaling-associated miRNAs, namely let-7 family miRNAs (i.e., let-7d-5p, let-7f-5p, let-7c, let-7e-5p), miR-103a-3p, miR-339-3p, miR-146a-5p, miR-23b-3p, miR-23a-3p, and miR-181a-5p, were differentially expressed following drug treatment. These differentially expressed miRNAs are circulating biomarker candidates that can be used to evaluate MOR stimulation and serve as novel clinical diagnostic tools for improving clinical outcomes.
📖 Papers frequently viewed together
20213.86Platelets
References35
Newest
#1Kaoru Toyama (Daiichi Sankyo)H-Index: 4
#2Hidetoshi FuruieH-Index: 4
Last. Hitoshi Ishizuka (Daiichi Sankyo)H-Index: 10
view all 4 authors...
Oxycodone is a semisynthetic opioid used for the treatment of moderate to severe pain. Two separate studies were conducted to assess the pharmacokinetic bioequivalence of a newly formulated oxycodone hydrochloride extended-release tablet to a marketed oxycodone product in Japan under fasting and fed conditions. Each study was a randomized, open-label, single-dose, single-center, two-period, two-way crossover study. Healthy male Japanese subjects received the oxycodone 10-mg products under fastin...
Source
#1Jin Wang (University of Texas MD Anderson Cancer Center)H-Index: 26
#2Jinyun Chen (University of Texas MD Anderson Cancer Center)H-Index: 19
Last. Subrata Sen (University of Texas at Austin)H-Index: 44
view all 3 authors...
MicroRNAs (miRNAs) are a group of small non-coding RNAs that are involved in regulating a range of developmental and physiological processes; their dysregulation has been associated with development of diseases including cancer. Circulating miRNAs and exosomal miRNAs have also been proposed as being useful in diagnostics as biomarkers for diseases and different types of cancer. In this review, miRNAs are discussed as biomarkers for cancer and other diseases, including viral infections, nervous s...
Source
#1Bastian FrommH-Index: 21
#2Tyler Billipp (Dartmouth College)H-Index: 1
Last. Kevin J. Peterson (Dartmouth College)H-Index: 55
view all 11 authors...
Although microRNAs (miRNAs) are among the most intensively studied molecules of the past 20 years, determining what is and what is not a miRNA has not been straightforward. Here, we present a uniform system for the annotation and nomenclature of miRNA genes. We show that less than a third of the 1,881 human miRBase entries, and only approximately 16% of the 7,095 metazoan miRBase entries, are robustly supported as miRNA genes. Furthermore, we show that the human repertoire of miRNAs has been sha...
Source
#1Ying Lu (Nantong University)H-Index: 6
#2De-Li Cao (Nantong University)H-Index: 18
Last. Yong-Jing Gao (Nantong University)H-Index: 41
view all 5 authors...
Abstract Glia-mediated neuroinflammation plays an important role in the pathogenesis of neuropathic pain. Our recent study demonstrated that TNF receptor associated factor-6 (TRAF6) is expressed in spinal astrocytes and contributes to the maintenance of spinal nerve ligation (SNL)-induced neuropathic pain. MicroRNA (miR)-146a is a key regulator of the innate immune response and was shown to target TRAF6 and reduce inflammation. In this study, we found that in cultured astrocytes, TNF-α, IL-1β, o...
Source
#1Kaoru Toyama (Daiichi Sankyo)H-Index: 4
#2Naoki Uchida (Showa University)H-Index: 9
Last. Shinichi Kobayashi (Showa University)H-Index: 4
view all 5 authors...
This single dose, open-label study investigated the safety, tolerability and pharmacokinetics of single oral doses of newly formulated immediate-release (IR) and hydrophilic matrix extended-release (ER) hydromorphone tablets in healthy Japanese subjects without co-administration of an opioid antagonist under fasting and fed conditions. Plasma and urinary concentrations of hydromorphone and metabolites were measured by liquid-chromatography tandem mass-spectroscopy. Following administration of th...
Source
#3Cristiana Cruceanu (Douglas Mental Health University Institute)H-Index: 27
Background Small ncRNAs (sncRNAs) offer great hope as biomarkers of disease and response to treatment. This has been highlighted in the context of several medical conditions such as cancer, liver disease, cardiovascular disease, and central nervous system disorders, among many others. Here we assessed several steps involved in the development of an ncRNA biomarker discovery pipeline, ranging from sample preparation to bioinformatic processing of small RNA sequencing data.
Source
#1Akhil Srivastava (OU: University of Oklahoma)H-Index: 16
#2Justyna FilantH-Index: 19
Last. Rajagopal Ramesh (OU: University of Oklahoma)H-Index: 57
view all 6 authors...
Exosomes are 30 - 100 nm bodies secreted from almost all types of cells into the extracellular spaces. They enclose in their lumen active genetic information in the form of messenger RNA (mRNA), micro RNA (miRNA), DNA and active peptides that are representative of the parental cell and can be isolated from different body fluids. Exosomes can participate in inter-cellular communication by trafficking molecules to their target cells. Because they can stably carry cargo including miRNA, mRNA, and p...
Source
#1Massmo BarbieratoH-Index: 1
#2Morena ZussoH-Index: 19
Last. Pietro Giusti (UNIPD: University of Padua)H-Index: 32
view all 4 authors...
MicroRNAs (miRNAs) are small non-coding RNAs that function as translational repressors and represent an important element in tissue development as well as disease. MiRNA sequences and their target sites in mRNAs show an extensive degree of conservation across species. A single miRNA is now believed to recognize the 3’untranslated region of mRNAs in a sequence-specific manner to inhibit the protein expression of literally hundreds of targeted mRNAs. The human genome encodes more than 1000 miRNAs ...
Source
#1Jin Lin (NCU: Nanchang University)H-Index: 9
#2Jing Li (NCU: Nanchang University)H-Index: 17
Last. Xiao-Zhong Wang (NCU: Nanchang University)H-Index: 16
view all 9 authors...
Exosomes are 30–120 nm endocytic membrane-derived vesicles that participate in cell-to-cell communication and protein and RNA delivery. Exosomes harbor a variety of proteins, nucleic acids, and lipids and are present in many and perhaps all bodily fluids. A significant body of literature has demonstrated that molecular constituents of exosomes, especially exosomal proteins and microRNAs (miRNAs), hold great promise as novel biomarkers for clinical diagnosis. In this minireview, we summarize rece...
Source
#1Chi XuH-Index: 8
#2Yue ZhangH-Index: 6
Last. Ping-Yee LawH-Index: 69
view all 5 authors...
The mechanism by which addictive drugs such as morphine regulate adult neurogenesis remains elusive. We now demonstrate that morphine can regulate neurogenesis by control of miR-181a and subsequent hippocampal neural progenitor cell (hNPC) lineages. In the presence of morphine, hNPCs preferentially differentiated into astrocytes, an effect blocked by the specific μ-opioid receptor antagonist, Cys2-Tyr3-Orn5-Pen7-amide. This effect was mediated by the Prox1/Notch1 pathway as demonstrated by an in...
Source
Cited By17
Newest
#1Logeeshan Velmanickam (University of Moratuwa)H-Index: 6
#2Vidura JayasooriyaH-Index: 4
Last. Dharmakeerthi Nawarathna (NDSU: North Dakota State University)H-Index: 10
view all 5 authors...
Dielectrophoresis is a well-understood phenomenon that has been widely utilized in biomedical applications. Recent advancements in miniaturization have contributed to the development of dielectrophoretic-based devices for a wide variety of biomedical applications. In particular, the integration of dielectrophoresis with microfluidics, fluorescence, and electrical impedance has produced devices and techniques that are attractive for screening and diagnosing diseases. This review article summarize...
Source
#1Austin Gowen (UNMC: University of Nebraska Medical Center)H-Index: 4
#2Katherine E. Odegaard (UNMC: University of Nebraska Medical Center)H-Index: 4
Last. Sowmya V. Yelamanchili (UNMC: University of Nebraska Medical Center)H-Index: 7
view all 7 authors...
Addiction is a chronic and relapsing brain disorder characterized by compulsive seeking despite adverse consequences. There are both heritable and epigenetic mechanisms underlying drug addiction. Emerging evidence suggests that non-coding RNAs (ncRNAs) such as microRNAs (miRNAs), long non-coding RNAs, and circular RNAs regulate synaptic plasticity and related behaviors caused by substances of abuse. These ncRNAs modify gene expression and may contribute to the behavioral phenotypes of addiction....
Source
#1Javier Cuitavi (University of Valencia)H-Index: 2
#2Lucía Hipólito (University of Valencia)H-Index: 15
Last. Meritxell Canals (University of Nottingham)H-Index: 47
view all 3 authors...
Opioid receptors (ORs) are undisputed targets for the treatment of pain. Unfortunately, targeting these receptors therapeutically poses significant challenges including addiction, dependence, tolerance, and the appearance of side effects, such as respiratory depression and constipation. Moreover, misuse of prescription and illicit narcotics has resulted in the current opioid crisis. The mu-opioid receptor (MOR) is the cellular mediator of the effects of most commonly used opioids, and is a proto...
Source
#1Ashish Kumar (Wake Forest Baptist Medical Center)H-Index: 2
#2S.D. Kim (Wake Forest Baptist Medical Center)H-Index: 6
Last. Gagan DeepH-Index: 41
view all 14 authors...
Abstract Background The United States is currently facing an opioid crisis. Novel tools to better comprehend dynamic molecular changes in the brain associated with the opioid abuse are limited. Recent studies have suggested the usefulness of plasma exosomes in better understanding CNS disorders. However, no study has ever characterized exosomes (small extracellular vesicles of endocytic origin) secreted by brain cells to understand the potential neurodegenerative effects of long-term oxycodone s...
Source
Source
#1Urszula Kosciuczuk (UMB: Medical University of Białystok)H-Index: 1
#2Paweł Knapp (UMB: Medical University of Białystok)H-Index: 8
Last. Anna M. Lotowska-Cwiklewska (UMB: Medical University of Białystok)H-Index: 1
view all 3 authors...
Opioids are the main group of pharmacological agents used during the perioperative period and provide a sedative and analgesic component. The observations of opioid consumption in West Europe indicate that this group of drugs is widely used in chronic noncancer pain therapy. Nearly 20 years ago, the first publications indicating that opioids, as an element of perioperative pharmacotherapy in oncologic patients, increase the risk of tumor recurrence and affect further prognosis were presented. Th...
Source
#1Richard J. Bodnar (QC: Queens College)H-Index: 66
Abstract This paper is the fortieth consecutive installment of the annual anthological review of research concerning the endogenous opioid system, summarizing articles published during 2017 that studied the behavioral effects of molecular, pharmacological and genetic manipulation of opioid peptides and receptors as well as effects of opioid/opiate agonists and antagonists. The review is subdivided into the following specific topics: molecular-biochemical effects and neurochemical localization st...
Source
#1Moonsang Seo (Great Ormond Street Hospital for Children NHS Foundation Trust)H-Index: 8
#2George P. AndersonH-Index: 111
BACKGROUND: Autism Spectrum Disorder (ASD) have long been conceived as a developmental disorder. A growing body of data highlights a role for alterations in the gut in the pathoetiology and/or pathophysiology of ASD. Recent work shows alterations in the gut microbiome to have a significant impact on amygdala development in infancy, suggesting that the alterations in the gut microbiome may act to modulate not only amygdala development but how the amygdala modulates the development of the frontal ...
Source
Migraine is a disabling and recurrent neurological disorder characterized by headache attacks that are often accompanied by sensory and motor disturbances. The value and importance of reliable biomarkers in migraine have been long recognized and a diverse range of biomarkers from biological samples to electrophysiological patterns and brain imaging has been proposed. There is still no consensus on specific biomarker(s) for migraine. Ideally, not a single but a battery of biomarkers would provide...
Source
BACKGROUND: The pathoetiology and pathophysiology of migraine are widely accepted as unknown. METHODS: The current article reviews the wide array of data associated with the biological underpinnings of migraine and provides a framework that integrates previously disparate bodies of data. RESULTS: The importance of alterations in stress- and pro-inflammatory cytokine- induced gut dysbiosis, especially butyrate production, are highlighted. This is linked to a decrease in the availability of melato...
Source
This website uses cookies.
We use cookies to improve your online experience. By continuing to use our website we assume you agree to the placement of these cookies.
To learn more, you can find in our Privacy Policy.