Gene

Metabolite

Biology

Comet assay

Reactive oxygen species

Zearalenone

Biochemistry

Glutathione

Food science

Intracellular

Oxidative phosphorylation

Oxidative stress

Antioxidant

Enzyme

Dichlorofluorescein

Pharmacology

Chemistry

Mycotoxin

DNA damage

Secondary metabolite

Toxicology in Vitro

Mycotoxin contamination of foods and feeds represent a serious problem worldwide. Zearalenone (ZEA) is a secondary metabolite produced by Fusarium species. This study explores oxidative cellular damage and intracellular defense mechanisms (enzymatic and non-enzymatic) in the hepatoma cell line HepG2 after exposure to ZEA and its metabolites (α-zearalenol, α-ZOL; β-zearalenol, β-ZOL). Our results demonstrated that HepG2 cells exposed to ZEA, α-ZOL or β-ZOL at different concentrations (0, 6.25, 12.5 and 25μM) showed: (i) elevated ROS levels (1.5- to 7-fold) based on the formation of the highly fluorescent 2',7'-dichlorofluorescein (DCF), (ii) increased DNA damage measured by the comet assay (9-45% higher), (iii) decreased GSH levels and CAT activity (decreased by 54%-25% and by 62%-25% for GSH and CAT, respectively) and (iv) increased GPx and SOD activities (increased by 50%-90% and by 26%-70%, respectively), compared to untreated cells. Our results suggest that mycotoxin-induced oxidative stress and damage may play a major role in the cytotoxic effects of ZEA and its metabolites. GSH and endogenous enzymes function together in protecting cells from ROS and the consequent damage after mycotoxin exposure. ZEA has a lower capacity to induce oxidative stress and damage in HepG2 cells than its metabolites at the tested concentrations.

Oxidative damage and disturbance of antioxidant capacity by zearalenone and its metabolites in human cells