Systematically characterize the absorbed effective substances of Wutou Decoction and their metabolic pathways in rat plasma using UHPLC-Q-TOF-MS combined with a target network pharmacological analysis.

Published on Jul 15, 2017in Journal of Pharmaceutical and Biomedical Analysis3.209
· DOI :10.1016/J.JPBA.2017.04.012
Tengfei Xu6
Estimated H-index: 6
(CAS: Chinese Academy of Sciences),
Shizhe Li6
Estimated H-index: 6
(JLU: Jilin University)
+ 4 AuthorsZhiqiang Liu20
Estimated H-index: 20
(CAS: Chinese Academy of Sciences)
Sources
Abstract
Abstract Wu-tou Decoction (WTD) is a famous traditional Chinese medicine (TCM) formula which is applied to treat arthritis and pain of joints. In this study, a sensitive and rapid method was developed for the separation and identification of the absorbed constituents and metabolites of WTD in the rats plasma by ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). A total of 22 absorbed prototype constituents and 49 metabolites were identified or tentatively characterized in dosed plasma. The possible metabolic pathways of these constituents involved sulfation, glucuronidation, demethylation, hydroxylation and so on. What’s more, we optimized the conventional process ways of network pharmacology and proposed a new concept called target-network pharmacology (T-NP). T-NP method used the absorbed constituents and the corresponding target proteins to generate compound-target network, and compare to the conventional method indifferent using the compounds collected from herbs, it could reduce the false positive results. We found that the following proteins were related to the WTD therapeutic effects, such as PTGS2, PTGS1, MAPK3, PPARG, TNF, IL4 and IL6. On the whole, the proposed method clearly presented the metabolic processes of WTD and the results gave a comprehensive metabolic profile of WTD in vivo for the first time. The combining use of the T-NP method could discover potential drug targets and disclose the biological processes of WTD, which will open up a new approach in the study of TCM in future.
📖 Papers frequently viewed together
37 Citations
6 Authors (Guangguo Tan, ..., Hong Wu)
5 Citations
5 Authors (Chen Qian, ..., Xiaohui Fan)
19 Citations
References26
Newest
#1Tengfei Xu (CAS: Chinese Academy of Sciences)H-Index: 6
#2Shu Liu (CAS: Chinese Academy of Sciences)H-Index: 18
Last. Zhiqiang Liu (CAS: Chinese Academy of Sciences)H-Index: 20
view all 6 authors...
The diterpenoid alkaloids as one type of heterocyclic alkaloids have been found in many traditional herbal medicines, such as genus Consolida, Aconitum, and Delphinium (Ranunculaceae). Pharmacological researches have indicated that many diterpenoid alkaloids are the main bioactive components which have analgesic, anti-inflammatory, anti-microbial, anti-tumor, cardiotonic, and anti-arrhythmic activities. Studies focused on the determination, quantitation and pharmacological properties of these al...
7 CitationsSource
#1Tengfei Xu (CAS: Chinese Academy of Sciences)H-Index: 6
#2Shu Liu (CAS: Chinese Academy of Sciences)H-Index: 18
Last. Zhiqiang Liu (CAS: Chinese Academy of Sciences)H-Index: 20
view all 7 authors...
The Wu-tou formula (WTF) is a Chinese medicine formula which has been applied to treat rheumatic arthritis (RA) and pain of joints for more than a thousand years. In this study, a pharmacodynamics combined urinary metabonomic study using UPLC-Q-TOF-HDMS was performed to assess the holistic efficacy of the Traditional Chinese Medicine (TCM) Wu-tou formula for treating RA in rats. Eighty male Sprague-Dawley rats were randomly divided into eight groups, named as the healthy control group (HG), the ...
16 CitationsSource
#1Jian Shi (Southern Medical University)H-Index: 13
#2Liang Zheng (Guangzhou University of Chinese Medicine)H-Index: 3
Last. Zhongqiu Liu (Southern Medical University)H-Index: 31
view all 10 authors...
Abstract Ethnopharmacological relevance Astragali radix is one of the well-known traditional Chinese herbal medicine, and possesses various biological functions, such as hepatoprotective and anticancer. In present study, to investigate the metabolism and pharmacokinetics of the major constituents of A. radix , a sensitive ultra-performance liquid chromatography–electrospray ionization–mass spectrometry (UPLC–MS/MS) method with shorter chromatographic running time was developed and validated for ...
55 CitationsSource
#1Bing Yu (ZJNU: Zhejiang Chinese Medical University)H-Index: 7
#2Yi Cao (ZJNU: Zhejiang Chinese Medical University)H-Index: 1
Last. Yao-Kang Xiong (ZJNU: Zhejiang Chinese Medical University)H-Index: 2
view all 3 authors...
Abstract Ethnopharmacological relevance Fuzi [the lateral root of Aconitum carmichaeli Debx (Ranunculaceae)] is a well-known traditional medicinal herb used to treat chronic heart failure (CHF). Aconitine-type alkaloids are major alkaloids that are responsible for the pharmacological activity and toxicity of this herb.To investigate therapeutic effects and pharmacokinetic profiles of aconitine-type alkaloids in CHF rats. Materials and methods The plasma pharmacokinetic profiles of aconitine, mes...
26 CitationsSource
#1Yanqiong Zhang (Peking Union Medical College)H-Index: 20
#2Song-song Wang (Capital Medical University)H-Index: 2
Last. Hongjun Yang (Peking Union Medical College)H-Index: 20
view all 8 authors...
Huanglian-Jie-Du decoction (HLJDD) is an important multiherb remedy in TCM, which is recently demonstrated to be effective to treat ischemic stroke. Here, we aimed to investigate the pharmacological mechanisms of HLJDD in the treatment of ischemic stroke using systems biology approaches. Putative targets of HLJDD were predicted using MetaDrug. An interaction network of putative HLJDD targets and known therapeutic targets for the treatment of ischemic stroke was then constructed, and candidate HL...
16 CitationsSource
#1Hong Kan (JLU: Jilin University)H-Index: 3
#2Wenyue Jiang (JLU: Jilin University)H-Index: 3
Last. Zhiqiang Liu (CAS: Chinese Academy of Sciences)H-Index: 54
view all 6 authors...
Abstract In this study, seven alkaloids were detected in Wu-tou decoction using ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC–MS n ). The aim of this study was to investigate the effect of Fritillariae Cirrhosae Bulbus , Fritillariae Thunbergii Bulbus , Pinelliae Rhizoma in different ratios with Wu-tou decoction (2:1, 1:1, 1:2) by measuring the therapeutic effects in Wu-tou decoction of main seven alkaloids including benzoylaconitine (BA), benzoylmesaconitin...
2 CitationsSource
#1Ning Zhao (Peking Union Medical College)H-Index: 9
#2Jian Li (Beijing University of Chinese Medicine)H-Index: 15
Last. Aiping Lu (Hong Kong Baptist University)H-Index: 59
view all 9 authors...
Molecular network-based analysis of Guizhi-Shaoyao-Zhimu decoction, a TCM herbal formula, for treatment of diabetic peripheral neuropathy
19 CitationsSource
Uncovering pharmacological mechanisms of Wu-tou decoction acting on rheumatoid arthritis through systems approaches: drug-target prediction, network analysis and experimental validation
65 CitationsSource
#1Chao WangH-Index: 7
#2Chunfang LiuH-Index: 13
Last. Na LinH-Index: 21
view all 14 authors...
Wu-tou decoction (WTD) is a classic traditional Chinese medicine formula and has been used effectively to treat joint diseases clinically. Previous reports indicated that WTD possesses anti-inflammatory activity; however, its actions on pain have not been clarified. Here, we investigated the antinociceptive activity of WTD in CFA-induced mice, and its possible mechanism of the action associated with transient receptor potential (TRP) ion channels was also explored. Our results showed that 1.58, ...
16 CitationsSource
#1Yao Qi (CAS: Chinese Academy of Sciences)H-Index: 6
#2Zifeng Pi (CAS: Chinese Academy of Sciences)H-Index: 19
Last. Zhiqiang Liu (CAS: Chinese Academy of Sciences)H-Index: 20
view all 7 authors...
Wu-tou-tang was applied to treat rheumatoid arthritis, rheumatic arthritis, and joint pain for thousands of years. Aconiti Radix Cocta is the primary component of Wu-tou-tang, and Aconiti Radix and Pinelliae Rhizoma is one of the famous combination taboos in traditional Chinese medicine. A urinary metabonomics method based on the ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) had been established to quantify the changes of the end...
6 CitationsSource
Cited By37
Newest
#1Kedi Liu (Shaanxi University of Chinese Medicine)H-Index: 1
#2Xingru Tao (Shaanxi University of Chinese Medicine)
Last. Tai-Wei Dong (Shaanxi University of Chinese Medicine)H-Index: 1
view all 13 authors...
Dalbergia Odorifera (DO) has been widely used for the treatment of cardiovascular and cerebrovascular diseasesinclinical. However, the effective substances and possible mechanisms of DO are still unclear. In this study, network pharmacology and molecular docking were used toelucidate the effective substances and active mechanisms of DO in treating ischemic stroke (IS). 544 DO-related targets from 29 bioactive components and 344 IS-related targets were collected, among them, 71 overlapping common...
Source
#1Yue-Hua Chen (LNUTCM: Liaoning University of Traditional Chinese Medicine)
#2Jing-Hua Bi (Shanxi Medical University)
Last. Hui-Peng Song (LNUTCM: Liaoning University of Traditional Chinese Medicine)
view all 10 authors...
Abstract null null The difficulty of traditional Chinese medicine (TCM) researches lies in the complexity of components, metabolites, and bioactivities. For a long time, there has been a lack of connections among the three parts, which is not conducive to the systematic elucidation of TCM effectiveness. To overcome this problem, a classification-based methodology for simplifying TCM researches was refined from literature in the past 10 years (2011-2020). The theoretical basis of this methodology...
1 CitationsSource
#1Kai Wang (Fourth Military Medical University)H-Index: 2
#2Lu Lei (Fourth Military Medical University)H-Index: 2
Last. Zhifu Yang (Fourth Military Medical University)H-Index: 10
view all 11 authors...
Source
Last. Jiansheng Li (Henan University)H-Index: 10
view all 7 authors...
Source
#1Li Mi (CPU: China Pharmaceutical University)
#2Yu-Chen Li (CPU: China Pharmaceutical University)
Last. Hua Yang (CPU: China Pharmaceutical University)H-Index: 20
view all 6 authors...
The tubers and roots of Aconitum (Ranunculaceae) are widely used as heart medicine or analgesic agents for the treatment of coronary heart disease, chronic heart failure, rheumatoid arthritis and neuropathic pain since ancient times. As a type of natural products mainly extracted from Aconitum plants, Aconitum alkaloids have complex chemical structures and exert remarkable biological activity, which are mainly responsible for significant effects of Aconitum plants. The present review is to summa...
Source
#1Jun Fu (Anhui University of Chinese Medicine)H-Index: 5
#2Huan Wu (Anhui University of Chinese Medicine)H-Index: 1
Last. Minghui Sun (Anhui University of Chinese Medicine)H-Index: 1
view all 5 authors...
Abstract Ethnopharmacological relevance Achyranthes bidentata Blume (AB) is a traditional Chinese medicine (TCM) widely used as a dietary supplement and anti-arthritis drug. Pharmacological studies have shown that Achyranthes bidentata Blume saponins (ABS) are the main bioactive ingredient. However, the metabolic profile and mechanisms of action of ABS against rheumatic arthritis (RA) remain to be established. Aim of the study: Our main objective was to investigate the metabolic profile and phar...
1 CitationsSource
#1Haijuan Zhi (CAS: Chinese Academy of Sciences)H-Index: 4
#2Zijia Zhang (CAS: Chinese Academy of Sciences)H-Index: 2
Last. Jinjun Hou (CAS: Chinese Academy of Sciences)H-Index: 17
view all 12 authors...
Abstract null null Salvianolic acids have a special synergic effect on panax notoginsenosides in acute myocardial infarction (AMI) and have been developed into a new drug as Danqi Tongmai Tablet (DQTT). To explore candidate targets and mechanisms of DQTT on AMI, a network pharmacology-based analysis was performed on absorbed prototype compounds of DQTT in rat plasma. Target prediction from network analysis indicated that the arachidonic acid pathway might contribute to the therapeutic effects of...
Source
#1Juan Li (ECUST: East China University of Science and Technology)H-Index: 1
#2Haiting WangH-Index: 4
Last. Yiping Du (ECUST: East China University of Science and Technology)H-Index: 22
view all 8 authors...
Although black tea and dark tea are both fermented teas, their fermentation processes are varied a lot and strongly affect their chemical components. The flavonoids which are the major active compounds in teas play an important role in their refreshing effect and health benefit. The differential flavonoid glycosides in these two fermented teas were investigated using nontargeted modification-specific metabolomics UHPLC–MS method. Principal component analysis (PCA) and orthogonal projection on la...
Source
#1Gao W (Peking Union Medical College)H-Index: 1
#2Nan SiH-Index: 12
Last. Hai-Yu ZhaoH-Index: 14
view all 10 authors...
Abstract Ethnopharmacological relevance Qingxin Lianzi Yin Decoction (QXLZY), a Chinese classical formula, has been widely used in the treatment of various chronic kidney diseases over 1,000 years. However, the current studies on QXLZY were mostly focused on its clinical efficacy, lacking systematic material basis research on constituents. Aim of the study This work aims to elucidate and quantify the chemical constituents, clarify the blood-absorbed components and excretion pathways, predict maj...
Source
#1Mohsen Vaez (Iranian Research Organization for Science and Technology)H-Index: 1
#2Seyed Javad Davarpanah (BMSU: Baqiyatallah University of Medical Sciences)H-Index: 7
The present study reports the in-vitro anticoagulant impact of acetonic extract (AE) of 42 lichen species, and the identification of potential bioavailable anticoagulant compounds from Umbilicaria decussata as a competent anticoagulant lichen species. Lichens' AEs were evaluated for their anticoagulant activity by monitoring activated partial thromboplastin time (APTT) and prothrombin time (PT) assays. A strong, positive correlation was observed between total phenolics concentration (TPC) of spe...
Source