Confocal/two-photon microscopy in studying colonisation of cancer cells in bone using xenograft mouse models.

Published on Dec 7, 2016in bonekey Reports
6.87
· DOI :10.1038/bonekey.2016.84
Gloria Allocca4
Estimated H-index: 4
(University of Sheffield),
Anjali P. Kusumbe19
Estimated H-index: 19
(University of Oxford)
+ 1 AuthorsNing Wang17
Estimated H-index: 17
(University of Sheffield)
Sources
Abstract
Confocal and two-photon microscopy has been widely used in bone research to not only produce high quality, three-dimensional images but also to provide valuable structural and quantitative information. In this article, we describe step-by-step protocols for confocal and two-photon microscopy to investigate earlier cellular events during colonisation of cancer cells in bone using xenograft mouse models. This includes confocal/two-photon microscopy imaging of paraformaldehyde fixed thick bone sections and frozen bone samples.
References30
Cited By5
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