Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells

Single-molecule localization microscopy (SMLM) is a rapidly evolving technique to resolve subcellular structures and single-molecule dynamics at the nanoscale. Here, we employ conventional BODIPY conjugates for live-cell SMLM via their previously reported red-shifted ground-state dimers (D II ), which transiently form through bi-molecular encounters and emit bright single-molecule fluorescence. We employ the versatility of D II -state SMLM to...