Accurate quantitation of mitochondrial DNA reveals uniform levels in human blastocysts irrespective of ploidy, age, or implantation potential

ObjectiveTo accurately determine mitochondrial DNA (mtDNA) levels in human blastocysts.DesignRetrospective analysis.SettingIVF clinic.Patient(s)A total of 1,396 embryos derived from 259 patients.Intervention(s)Blastocyst-derived trophectoderm biopsies were tested by next-generation sequencing (NGS) and quantitative real-time polymerase chain reaction (qPCR).Main Outcome Measure(s)For each sample the mtDNA value was divided by the nuclear DNA...