E. coli metabolic engineering for gram scale production of a plant-based anti-inflammatory agent

In this report, the heterologous production of salicylate (SA) is the basis for metabolic extension to salicylate 2- O - β - d -glucoside (SAG), a natural product implicated in plant-based defense mechanisms. Production was optimized through a combination of metabolic engineering , gene expression variation, and co-culture design. When combined, SA and SAG production titers reached ~0.9 g/L and ~2.5 g/L, respectively. The SAG compound was then...