Deferoxamine immobilized poly(D,L-lactide) membrane via polydopamine adhesive coating: The influence on mouse embryo osteoblast precursor cells and human umbilical vein endothelial cells.
Osteogenesis and angiogenesis play the prominent role in the bone regeneration. In this study, deferoxamine (DFO), an induced agent for osteogenesis and angiogenesis, was modified onto the surface of poly(D,L-lactide) (PDLLA) membrane via a facile and convenient approach based on the self-polymerization of dopamine (DOPA). The surface composition, morphology, hydrophilicity and surface energy of the original and modified PDLLA membranes were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), scanning electronic microscopy (SEM), atomic force microscopy (AFM) and contact angle measurement. The surface roughness and hydrophilicity of the PDLLA membrane were obviously increased by introducing either the single polydopamine (PDOPA) or the dual layers of PDOPA and DFO. In vitro cells culture experiments indicated that both the PDLLA/PDOPA and PDLLA/PDOPA-DFO composite membranes were more beneficial to the attachment, proliferation and spreading of MC3T3-E1 cells and HUVECs compared to the original PDLLA membrane. The PDLLA/PDOPA-DFO membrane was supportive for the proliferation of both MC3T3-E1 cells and HUVECs, and especially for HUVECs. The results suggested that the as-prepared PDLLA/PDOPA-DFO composite can be expected to be used as a promising bone regenerative material with promoted angiogenesis.
Abstract Dental implants made of pure titanium suffer from abrasion and scratch during routine oral hygiene procedures. This results in an irreversible surface damage, facilitates bacteria adhesion and increases risk of peri-implantitis. To overcome these problems, titanium nitride (TiN) coating was introduced to increase surface hardness of pure titanium. However, the osteoconductivity of TiN is considered to be similar or superior to that of titanium and its alloys and therefore surface modifi...
Abstract The aim of the research presented in the paper was to evaluate the feasibility of using hydrophobic preparations based on organosilicon compounds for protection treatment on the hybrid fiber reinforced ultra-high performance concrete (FRUHPC) surface. Three polisiloxanes agents were deposited onto the seven types of concretes, with a steel-polypropylene fiber content ranging from 0% to 1%. In this investigation, the surface free energy (SFE) of the coatings was calculated using the Owen...
#1Alina Freiman(Technion – Israel Institute of Technology)H-Index: 6
#2Yulia Shandalov(Technion – Israel Institute of Technology)H-Index: 12
Last. Shulamit Levenberg(Technion – Israel Institute of Technology)H-Index: 41
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Background Adipose-derived mesenchymal stem cells (MSCs) have been gaining fame mainly due to their vast clinical potential, simple isolation methods and minimal donor site morbidity. Adipose-derived MSCs and microvascular endothelial cells have been shown to bear angiogenic and vasculogenic capabilities. We hypothesized that co-culture of human adipose-derived MSCs with human adipose-derived microvascular endothelial cells (HAMECs) will serve as an effective cell pair to induce angiogenesis and...
Background Multiple sclerosis is a demyelinating disease mostly of autoimmune origin that affects and damages the central nervous system, leading to a disabling condition. The aim of the present study was to investigate whether administration of mesenchymal stem cells from human periodontal ligament (hPDLSCs) could ameliorate multiple sclerosis progression by exerting neuroprotective effects in an experimental model of autoimmune encephalomyelitis (EAE).
#2Chuang Luo(MOE: Chinese Ministry of Education)H-Index: 1
Last. Changren Zhou(JNU: Jinan University)H-Index: 37
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Abstract To develop a chitooligosaccharide(COS)-functionalized poly( d,l -lactide) (PDLLA) membrane to enhance growth and osteogenic differentiation of MC3T3-E1 cells, firstly a thin polydopamine (PDOPA) layer was adhered to the PDLLA membrane via the self-polymerization and strong adhesion behavior of dopamine. Subsequently, COS was immobilized covalently on the resultant PDLLA/PDOPA composite membrane by coupling with PDOPA active coating. The successful immobilization of the PDOPA and COS was...
Electrospinning is a versatile technique to generate large quantities of micro- or nano-fibers from a wide variety of shapes and sizes of polymer. The aim of this study is to develop functionalized electrospun nano-fibers and use a mussel-inspired surface coating to regulate adhesion, proliferation and differentiation of human adipose-derived stem cells (hADSCs). We prepared poly(lactic acid) (PLA) fibers coated with polydopamine (PDA). The morphology, chemical composition, and surface propertie...
Abstract Thin radio-frequency magnetron sputter deposited nano-hydroxyapatite (HA) films were prepared on the surface of a Fe-tricalcium phosphate (Fe-TCP) bioceramic composite, which was obtained using a conventional powder injection moulding technique. The obtained nano-hydroxyapatite coated Fe-TCP biocomposites (nano-HA-Fe-TCP) were studied with respect to their chemical and phase composition, surface morphology, water contact angle, surface free energy and hysteresis. The deposition process ...
Abstract Regeneration of healthy endothelium onto vascular graft materials is imperative for prevention of intimal hyperplasia and thrombogenesis. In this study, we investigated the effect of collagen type IV (COL-IV) immobilized onto electrospun nanofibers on modulation of endothelial cell (EC) function, as a potential signal to rapid endothelialization of vascular grafts. COL-IV is assembled in basement membrane underneath intimal layer and regulates morphogenesis of blood vessels. For immobil...
#2A.G. Martins(UC: University of Coimbra)H-Index: 2
Abstract The hydroxyapatite surface characterization may be important regarding its reactivity in the synthesis of composites and in the context of the multiple interactions with the biological environment, after in vivo implantation, including protein adhesion and cell attachment on the mineral surface. In this work, the surface properties of a bone-derived hydroxyapatite with ca. 3.5 wt% of carbonate content (named as carbonated) and a hydroxyapatite obtained by calcination of the carbonated o...
For bioabsorbable vascular scaffolds (BVS), thrombosis is an important clinical problem. Poly(lactic acid) (PLA), as a commonly used manufacturing material of BVS, is always facing thrombosis event...
#2Tianwen Xin(Soochow University (Taiwan))H-Index: 3
Last. Wenguo Cui(SJTU: Shanghai Jiao Tong University)H-Index: 53
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Abstract Bone regeneration is a complex process consisting of three major parts, osteogenesis, angiogenesis, and antibacterial. The absence of any parts will hinder the regeneration process. For now, there are still limited researches on preparing a general platform for properly combining these three parts. Here, we proposed a strategy by using injectable 4-arm-polyethylene glycol-thiol (4-arm-PEG-SH) hydrogel incorporated with liposomes-calcium phosphate nanoparticles (Lip#CaP) to synthesized o...
Last. Zhi Ping Xu(UQ: University of Queensland)H-Index: 83
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Continuous blood purification technology such as hemodiafiltration has been used worldwide for saving patients suffering from severe diseases or organ function failure, especially in the intensive care unit and emergency setting. The filters as core devices are commonly made of polymer materials as hollow fiber membranes. However, the membrane is often inductively blocked by blood clot formation due to its interactions with blood components. Heparin is the anticoagulant often used in clinical pr...
: A method to mediate biomineralization of electrospinning poly(L-lactide) (PLLA) fibrous membrane assisted by polydopamine (PDA) coating was developed to obtain enhanced osteoconductive activity. The biomineralization mechanism, surface composition, morphology and hydrophilicity of the original and modified PLLA fibrous membranes were characterized. Results revealed that the PDA coating effectively accelerated the formation of hydroxyapatite (HA) on PLLA fibrous membrane and resulted a great in...
Last. Binghong Luo(JNU: Jinan University)H-Index: 16
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Abstract In order to better utilize and combine the advantages of poly( d , l -lactide) (PDLLA) matrix and natural polysaccharide chitin whiskers (CHWs), needle-like CHWs with chiral nematic liquid crystal characteristics and remarkable moduli were chosen to surface modify PDLLA film. By a vertical coating method, a stable and well-ordered CHWs coating was formed on the surface of the PDLLA film through a polydopamine (PDA) interlayer. The high stability of the well-ordered CHWs coating on the s...
#1Ming-Gene Tu(PRC: China Medical University (PRC))H-Index: 15
#2Chia-Che Ho(PRC: China Medical University (PRC))H-Index: 14
Last. Ming-You Shie(PRC: China Medical University (PRC))H-Index: 29
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Abstract Introduction This research was intended to evaluate the feasibility of mineral trioxide aggregate (MTA) powder coated with polydopamine (PDA) in dental and bone tissue regeneration by investigating the hydration, physicochemical properties, and biological performance of hydrated cements. Methods The MTA powder was well suspended in a dopamine solution buffered at a pH of 8.5 using tris(hydroxymethyl)aminomethane buffer and vigorously stirred for 12 hours at room temperature. The PDA-coa...
Last. Changren Zhou(JNU: Jinan University)H-Index: 37
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Abstract To synergistically improve the mechanical properties and osteogenic activity of electrospinning poly( l -lactide) (PLLA) membrane, chitosan (CS) nanofibers were firstly introduced to prepare sub-micro and nanofibers interpenetrated PLLA/CS membrane, which was further surface modified with a polydopamine (PDA) layer to obtain PLLA/CS-PDA. Surface morphology, porosity, surface area and hydrophilicity of the obtained fibrous membranes were studied in detail. As compared to pure PLLA, the s...
Last. Changren Zhou(JNU: Jinan University)H-Index: 37
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Abstract In this study, icariin (ICA), one of the main active ingredients of Herba Epimedii for osteogenesis, was applied to functionalize electrospinning poly( l -lactide) (PLLA) fibrous membrane via an intermediate layer of polydopamine (PDA) to obtain enhanced cytocompatibility and osteogenic activity. For this purpose, an array of PDA-coated PLLA fibrous membranes (PLLA-0.5PDA, PLLA-1PDA, PLLA-2PDA, PLLA-5PDA) and ICA-modified PLLA-2PDA fibrous membranes (PLLA-2PDA-10ICA, PLLA-2PDA-20ICA, PL...