The State of Cell Blocks and Ancillary Testing: Past, Present, and Future.

Published on Aug 24, 2016in Archives of Pathology & Laboratory Medicine5.534
路 DOI :10.5858/ARPA.2016-0125-RA
Anjali Saqi22
Estimated H-index: 22
(CUMC: Columbia University Medical Center)
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Abstract
Cell blocks are an integral part of cytology, but their utility is recognized probably more now than ever before, largely owing to the significant role they play in ancillary testing, particularly ...
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References35
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#1Ronald BalassanianH-Index: 15
#2Geoffrey D. WoolH-Index: 13
Last. Martha B. Pitman (Harvard University)H-Index: 55
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BACKGROUND Cell block (CB) techniques for fine-needle aspiration biopsies (FNABs) vary. A direct comparison of CB techniques with statistical validation was performed to identify the best method. METHODS Three CB techniques were compared: 1) FNAB rinsed in saline and clotted with plasma and thrombin (SPT); 2) FNAB rinsed in formalin and clotted with HistoGel (HG); and 3) FNAB rinsed in formalin, centrifuged, and the pellet captured in a collodion bag (ColB). FNAB was performed on 35 random surgi...
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#1Wook Youn Kim (Konkuk University)H-Index: 12
#2Seo Young Oh (Konkuk University)H-Index: 9
Last. Tae Sook Hwang (Konkuk University)H-Index: 18
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In fine needle aspiration biopsy (FNAB) of thyroid nodules, LBC is adopted in most of the hospitals and clinics in Korea for its convenience. BRAF mutation test has been introduced as an important ancillary test, but its applicability has not been completely proven with LBC samples.Five aspirates from thyroidectomy specimens were simultaneously processed into LBC and CS slides, in which BRAF mutation tests were performed using three primer sets with PCR products of 72, 164, and 226 base pairs (b...
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The results from molecular assays can be affected significantly by the preanalytic condition of cytologic samples. The authors review current knowledge on the use of cytologic samples for epidermal growth factor receptor (EGFR) mutation testing in non鈥搒mall cell lung cancer with a focus on preanalytic parameters. A systematic electronic search of the MEDLINE database was performed to identify original articles that reported the use of cytologic samples for EGFR molecular analysis and included a ...
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#1Sinchita Roy-Chowdhuri (University of Texas MD Anderson Cancer Center)H-Index: 30
#2Rashmi S. Goswami (University of Texas MD Anderson Cancer Center)H-Index: 14
Last. John Stewart (University of Texas MD Anderson Cancer Center)H-Index: 32
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BACKGROUND The use of cytology specimens for next-generation sequencing (NGS) is particularly challenging because of the unconventional substrate of smears and the often limited sample volume. An analysis of factors affecting NGS testing in cytologic samples may help to increase the frequency of successful testing. METHODS This study reviewed variables associated with all in-house cytology cases (n鈥=鈥207) that were analyzed by NGS with the Ion Torrent platform during a 10-month interval. A stati...
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Liquid-based cytology (LBC) has been progressively used for evaluating fine needle aspiration (FNA) specimens. However, limited studies have examined LBC in FNA of parathyroid lesions. We retrospectively reviewed 24 FNA specimens of parathyroid lesions, including 6 specimens prepared by conventional smear, 12 specimens prepared using ThinPrep method, and 6 specimens prepared using SurePath method. The 18 LBC specimens were also used for cell block preparation and immunostaining for parathyroid h...
#1Shuko HaradaH-Index: 19
Last. Isam A. EltoumH-Index: 54
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BACKGROUND In the era of personalized medicine, requests for molecular testing of specimens obtained with minimally invasive procedures such as fine-needle aspiration have been increasing. Although cell blocks (CBs) are the recommended specimens for molecular testing, their performance has not been well analyzed. The objective of this study was to assess the frequency and types of samples deemed unsatisfactory for molecular testing (quantity not sufficient [QNS]). METHODS One year after the impl...
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#1Ossama Tawfik (KU: University of Kansas)H-Index: 48
#2Marilyn Davis (KU: University of Kansas)H-Index: 6
Last. Fang Fan (KU: University of Kansas)H-Index: 20
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Objective: To date, the impact of digital imaging on routine cytology remains far from perfect. Cellblock (CB) preparations from Pap samples have been shown to be diagnostically valuable. We evaluated the validity of utilizing whole-slide imaging (WSI) prepared from Pap CBs as a screening tool. Study Design: A total of 1,110 CB slides prepared from residual Pap samples were analyzed - 563 normal, 282 atypical squamous cells of undetermined significance (ASCUS), 12 atypical squamous cells-cannot ...
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#1John P. Crapanzano (CUMC: Columbia University Medical Center)H-Index: 9
#2Anjali Saqi (CUMC: Columbia University Medical Center)H-Index: 22
Greater numbers of minimally invasive procedures are being performed to diagnose, stage, and/or treat lung cancer. These procedures pose less risk to patients but greater challenges for pathologists and clinicians alike. Specimens derived from minimally invasive procedures are small and comprised of fine-needle aspirates and/or core biopsies. Standardized criteria have been developed for classifying small biopsies and cytology specimens and performing molecular testing. However, similar protocol...
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#1Anjali Saqi (CUMC: Columbia University Medical Center)H-Index: 22
#2John P. Crapanzano (CUMC: Columbia University Medical Center)H-Index: 9
There is a shift in the diagnosis and treatment of lung carcinomas. Identification of the subtype of non-small cell lung cancers (NSCLCs) and underlying mutations of lung adenocarcinomas drives management. This has coincided with the performance of greater numbers of minimally invasive procedures and the acquisition of small specimens, including fine needle aspirations (FNAs) and core biopsies, which frequently represent the only tissue amenable or attainable for both diagnosis and ancillary tes...
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#1S. Prendeville (Cork University Hospital)H-Index: 1
#2T. Brosnan (Cork University Hospital)H-Index: 1
Last. J. McCarthy (Cork University Hospital)H-Index: 1
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Objective Cytoblocks (CBs), or cell blocks, provide additional morphological detail and a platform for immunocytochemistry (ICC) in cytopathology. The Cellient鈩 system produces CBs in 45聽minutes using methanol fixation, compared with traditional CBs, which require overnight formalin fixation. This study compares Cellient and traditional CB methods in terms of cellularity, morphology and immunoreactivity, evaluates the potential to add formalin fixation to the Cellient method for ICC studies and ...
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#1Savitri Krishnamurthy (University of Texas MD Anderson Cancer Center)H-Index: 63
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Optical imaging modalities are emerging as digital microscopy tools for tissue examination. The investigation of these techniques for potential applications in anatomic pathology practice has focused primarily on surgical pathology and has not included cytopathological specimens. We evaluated the feasibility of using digital confocal microscopy (CM) to examine cytopathological specimens. Smears and cell suspensions collected in RPMI solution were prepared from tissue scrapes obtained from surgic...
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#1Erkka TommolaH-Index: 1
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(1) Background: The Milan System for Reporting Salivary Gland Cytopathology (MSRSGC) was introduced in 2018, bringing an organ-specific classification system for salivary gland cytopathology. The aim of present study is to evaluate the MSRSGC prospectively, based on a two-year experience in the tertiary care center pathology department, and evaluate the role of routine cell block (CB) preparation in salivary gland cytopathological diagnostics. (2) Methods: In our institution, the Department of P...
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#1Susan Shyu (Johns Hopkins University)H-Index: 1
#2Ankit Rajgariah (Johns Hopkins University)H-Index: 1
Last. Syed Z. Ali (Johns Hopkins University)H-Index: 43
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Abstract Introduction While the diagnostic utility of lymph node fine needle aspiration (FNA) is well established in the evaluation of metastatic malignancy, its value in the diagnosis of lymphoma is more controversial; yet, there is a growing trend among practitioners towards less invasive procedures such as FNA and core needle biopsy (CNB). The guidelines recently published by the ASCP/CAP regarding the workup of lymphoma include recommendations on the value and limitations of FNA. Materials a...
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Abstract Introduction Thyroid fine needle aspiration (tFNA) is a powerful screening tool for assessing solitary thyroid nodules. Generally, morphologic evaluation of smears yields an accurate diagnosis; but, in some cases it is useful to have a cell block (CB) to conduct ancillary studies such as immunohistochemistry (IHC). Cytologic diagnoses guide clinical decisions, so it is important that accurate and efficient diagnoses be rendered. Our study evaluates the diagnostic utility of the CB in th...
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BACKGROUND Currently, the golden rule for the diagnosis of urothelial carcinoma is biopsy and cystoscopy, unfortionally both are costly, invasive, and uncomfortable. While most urothelial cancers are noninvasive at presentation, it is necessary to find a highly sensitive, noninvasive way to diagnose in its earlier stages, Cytology with immunostaining is a noninvasive, reliable method that might play a role in detecting the earlier stages before its progression and accurate correlation with diffe...
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#1Ana Maria Cretu (Ovidius University)
Last. Anca Antonela Nicolau (Ovidius University)H-Index: 1
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#1Kaveri Hallikeri (Sri Dharmasthala Manjunatheshwara College of Dental Sciences & Hospital)H-Index: 8
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Objectives null To determine the role and efficacy of fine needle aspiration cytology (FNAC) and cell block in diagnosis of jaw lesions and compare the agreement between FNAC and cell block to predict the diagnosis. null Method null The sample comprised 51 cases, including 12 odontogenic keratocysts (OKCs), 8 ameloblastomas, 22 radicular cysts, 7 dentigerous cysts, and 1 each of intraosseous mucoepidermoid carcinoma (MEC) and adenomatoid odontogenic tumor (AOT). FNAC samples remaining after hema...
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#2Roope Huhtamella (UTA: University of Tampere)H-Index: 1
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INTRODUCTION Thyroid cytology is a widely accepted tool in the clinical triaging of nodular lesions. Cell blocks (CBs) can help in the diagnosis of atypical lesions, namely, thyroid Bethesda category of Atypia of undetermined significance/follicular lesion of undetermined significance (AUS/FLUS). METHODS In a series of 224 AUS/FLUS thyroid samples with CB, we studied CB cellularity and feasibility of 3 immunohistochemical markers (cytokeratin 19 [CK19], HBME-1, and galectin-3) apart and in combi...
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#1Maverick Melega (UNITO: University of Turin)H-Index: 2
#2Marta Santos (University of Porto)H-Index: 15
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BACKGROUND Cell blocks are alternative preparations of fluid cytological specimens. They can be used for immunochemical studies as complementary tools or when other techniques (eg, immunocytochemistry, flow cytometry) are not available. OBJECTIVES We aimed to provide comparative morphologic, immunohistochemical, and technical features of agar-based cell blocks (ACBs) and cell tube blocks (CTBs) from cavitary effusions. METHODS Agar-based cell blocks and CTBs were obtained from canine and feline ...
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#1Jonas J. Heymann (NewYork鈥揚resbyterian Hospital)H-Index: 12
#2Lorene Yoxtheimer (Tulane University)H-Index: 2
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BACKGROUND: Morphologic and genetic analysis of thyroid nodules may be performed from a single vial. Preanalytic variables that affect nucleic acid extracted from a single vial are evaluated. METHODS: Thyroid fine-needle aspiration (FNA) specimens collected in CytoLyt were evaluated. A ThinPrep slide was prepared. Extracted nucleic acids were analyzed using Oncomine Comprehensive Panel, version 2, after Ion AmpliSeq library preparation. A pathologist and a cytotechnologist enumerated specimen ce...
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