Glial and neuronal cellular changes in the glaucomatous human retina

S. Thanos1
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J. M. Rorbach1
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H.-J. Thiel1
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Abstract
: The purpose of the present study was analysis of the cellular response to glaucoma in the human retina. Retinal strips obtained from two enucleated eyes with therapy-resistant, absolute glaucoma were explanted and cultured in vitro. The morphology of the remaining cell populations was assessed with the DiI method in non-cultured, formalin-fixed retinal tissue from both retinas. We found immunohistochemically identifiable glia cells migrating out from the explants and glial processes formed on the substrate. Labelling of representative retinal areas with the fluorescent dye DiI applied to the nerve fiber layer resulted in delineation of growth cone-bearing glial processes, of occasional non-atrophied ganglion cells, and of amacrines and horizontal cells in deeper layers of the retina. The results demonstrate that glaucoma leads both to selective damage of ganglion cells and to a glial proliferation characterized by the formation of processes both in the retina and following explantation.
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Purpose: The purpose of this research was to investigate the effect of glaucoma-related insults, specifically oxygen and/or glucose deprivation (OGD) on the survival and genes expression of human Muller cells (MIO-M1), and retinal ganglion cells (RGCs) using the human organotypic retinal culture (HORC) model. Methods: MIO-M1 cells and HORCs were exposed to different levels of OGD using a custom-built chamber to control oxygen levels. Cell survival was evaluated using MTS and LDH assays while RGC...
PURPOSE. The present study was conducted to examine whether the morphology of the retinal ganglion cells is altered in advanced glaucoma. Perikaryal, axonal, and dendritic alterations were monitored in glaucoma-resistant retinal ganglion cells by postvitam application of the fluorescent dye DiI. METHODS. The retinas of four amaurotic glaucomatous eyes and four normal eyes enucleated after death were used in this study. The retinas were freed from surrounding tissue, prepared as flatmounts on a n...
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