ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
Abstract
The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First, a 1-kb ssODN co-injected with guide RNA (gRNA) and Cas9 messenger RNA produce GFP-KI at the rat Thy1 locus. Then, two gRNAs with two 80-bp ssODNs...
Paper Details
Title
ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
Published Date
Jan 20, 2016
Journal
Volume
7
Issue
1
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