Original paper
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
Abstract
Genome-wide sgRNA libraries based on rules for on-target activity improve results of Cas9-based screens and facilitate a further refinement of on- and off-target prediction algorithms. CRISPR-Cas9–based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off-target...
Paper Details
Title
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
Published Date
Jan 18, 2016
Journal
Volume
34
Issue
2
Pages
184 - 191
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Notes
History