A Vapor Exposure Model for Neonatal Mice

Published on Jan 1, 2002in Toxicology Mechanisms and Methods2.295
· DOI :10.1080/15376510209167936
Dana R. Anderson25
Estimated H-index: 25
(United States Army Medical Research Institute of Chemical Defense),
Larry W. Mitcheltree4
Estimated H-index: 4
(United States Army Medical Research Institute of Chemical Defense)
+ 3 AuthorsMark B. Gold4
Estimated H-index: 4
(United States Army Medical Research Institute of Chemical Defense)
Sulfur mustard (HD) is a vesicant compound that was first used as a chemical warfare agent in World War I. (Papirmeister et al. 1991). Numerous animal models have been used to study HD-induced vesication. In this article, we describe modifications of the vapor cup model of Mershon and colleagues (1990) to establish a new vapor cup model for use in neonatal mice. The need to develop this model resulted from the development of gene-targeted knockout mice that can be used to evaluate the function of specific genes and their contribution to HD-induced pathology. However, the knockouts are haired mice; therefore, it is necessary to perform vapor exposures on the pups prior to their growing hair. Neonatal mice were anesthetized with isofluorane inhalation and placed in sternal recumbency on a 37°C isothermal pad to maintain body heat during exposure. The vapor cup consisted of a 1.5-mL microfuge tube cap (8 mm inside diameter) modified using a Dremel tool to contour its rim to better fit the curve of a mouse pu...
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