Measurement of cell proliferation by labeling of DNA with stable isotope-labeled glucose: Studies in vitro , in animals, and in humans

Volume: 95, Issue: 2, Pages: 708 - 713
Published: Jan 20, 1998
Abstract
A method for measuring DNA synthesis and, thus, cell proliferation, in vivo is presented. The technique consists of administering [6,6-2H2]Glc or [U-13C]Glc, isolating genomic DNA, hydrolyzing enzymatically to free deoxyribonucleosides, and derivatizing for GC-MS analysis of dA or dG isotopic enrichments, or both. Comparison of dA or dG to extracellular Glc enrichment (with a correction for intracellular dilution) reveals the fraction of newly...
Paper Details
Title
Measurement of cell proliferation by labeling of DNA with stable isotope-labeled glucose: Studies in vitro , in animals, and in humans
Published Date
Jan 20, 1998
Volume
95
Issue
2
Pages
708 - 713
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