Evaluation of differentially expressed genes identified in keratoconus.
Abstract
To identify the differentially expressed genes (DEGs) in the human keratocytes in keratoconus.Total RNA extracted from cultured corneal stromal fibroblasts from normal and keratoconic corneas were used for the synthesis of cDNA. DEGs were screened by an annealing control primer(TM)-based PCR method using GeneFishing() DEG kits. The differentially expressed bands were sequenced and analyzed. The genes identified were further evaluated by reverse...
Paper Details
Title
Evaluation of differentially expressed genes identified in keratoconus.
Published Date
Nov 28, 2009
Journal
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