Cell therapy as part of the concept of regenerative medicine represents an upcoming platform technology. Although cultured epidermal cells have been used in burn treatment for decades, new developments have renewed the interest in this type of treatment. Whereas early results were hampered by long culture times in order to produce confluent sheets of keratinocytes, undifferentiated proliferating cells can nowadays be applied on burns with different application techniques. The application of cells on carriers has improved early as well as long-term results in experimental settings. The results of several commercially available epidermal substitutes for burn wound treatment are reviewed in this article. These data clearly demonstrate a lack of randomized comparative trials and application of measurable outcome parameters. Experimental research in culture systems and animal models has demonstrated new developments and proof of concepts of further improvements in epidermal coverage. These include combinations...
Last. Qizhou Lian(HKU: University of Hong Kong)H-Index: 29
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Mesenchymal stem cells (MSCs) are one of a few stem cell types to be applied in clinical practice as therapeutic agents for immunomodulation and ischemic tissue repair. In addition to their multipotent differentiation potential, a strong paracrine capacity has been proposed as the principal mechanism that contributes to tissue repair. Apart from cytokine/chemokine secretion, MSCs also display a strong capacity for mitochondrial transfer and microvesicle (exosomes) secretion in response to injury...
Tissue engineering uses cells, signaling molecules, and/or biomaterials to regenerate injured or diseased tissues. Ex vivo expanded mesenchymal stem cells (MSC) have long been a cornerstone of regeneration therapies; however, drawbacks that include altered signaling responses and reduced homing capacity have prompted investigation of regeneration based on endogenous MSC recruitment. Recent successful proof-of-concept studies have further motivated endogenous MSC recruitment-based approaches. Ste...
Vascularization is one of the great challenges that tissue engineering faces in order to achieve sizeable tissue and organ substitutes that contain living cells. There are instances, such as skin replacement, in which a tissue-engineered substitute does not absolutely need a preexisting vascularization. However, tissue or organ substitutes in which any dimension, such as thickness, exceeds 400 μm need to be vascularized to ensure cellular survival. Consistent with the wide spectrum of approaches...
#2Oh Sang Kwon(UPenn: University of Pennsylvania)H-Index: 30
#3Zhikun Zhang(UPenn: University of Pennsylvania)H-Index: 1
Last. George Cotsarelis(UPenn: University of Pennsylvania)H-Index: 56
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Humans lack robust regeneration of hair follicles after skin wounding. George Cotsarelis and colleagues now show that γδ T cells are not present at high levels in human skin, that in mice they are a key initial source of the protein fibroblast growth factor 9 and that this factor modulates hair follicle regeneration during skin wound healing. These results suggest a possible topical clinical treatment to regrow hair after wounding and perhaps for other conditions of hair loss.
#2Kaari A. Lynch(UC: University of Cincinnati)H-Index: 6
Last. Steven T. Boyce(UC: University of Cincinnati)H-Index: 62
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Generation of skin appendages in engineered skin substitutes has been limited by lack of trichogenic potency in cultured postnatal cells. To investigate the feasibility and the limitation of hair regeneration, engineered skin substitutes were prepared with chimeric populations of cultured human keratinocytes from neonatal foreskins and cultured murine dermal papilla cells from adult GFP transgenic mice and grafted orthotopically to full-thickness wounds on athymic mice. Non-cultured dissociated ...
Objective: To investigate the feasibility and safety of human bone marrow mesenchymal stem cells (BM-MSCs) transplantation on the improvement of burn wound healing. Method: Human BM-MSCs were injected into the skin of the mouse models, and the new blood vessels growth, the engraftment of BM-MSCs and the speed of healing were observed. Moreover the body weight and activity were tested after BM-MSCs transplantation. Results: We found that wound surface healing was significantly accelerated when BM...
Burns not only destroy the barrier function of the skin but also alter the perceptions of pain, temperature, and touch. Different strategies have been developed over the years to cover deep andextensiveburnswiththeultimategoalofregeneratingthebarrierfunctionoftheepidermis while recovering an acceptable aesthetic aspect. However, patients often complain about al oss of skin sensation and even cutaneous chronic pain. Cutaneous nerve regeneration can occur from the nerve endings of the wound bed, b...
Abstract Bacterial contamination remains a constant threat in burn wound care. Topical treatments to combat contaminations have good bactericidal effects but can have detrimental effects for the healing process. Treatments with for example silver can increase healing times. Honey based products can be a good alternative as it is antibacterial and patient-friendly. We evaluated the bactericidal and cytotoxic effects of a honey based gel and silver sulphadiazine in a human burn wound model with Ps...
OBJECTIVES: To determine the effectiveness of HP802-247 compared with bacitracin ointment in healing wounds resulting from Mohs micrographic surgery. METHODS: Open-label, randomized pilot study conducted at a single center. Subjects were randomized to either HP802-247 (5M cells/mL) applied weekly or bacitracin ointment applied daily. Treatment continued for up to 12 weeks or complete wound closure. Primary efficacy was effectiveness as measured by the Investigator's Global Assessment of Healing ...
Nickel is a ubiquitous trace element and the commonest cause of metal allergy among the people. Nickel allergy is a chronic, recurring problem; females are affected more commonly than males. Nickel allergy may develop at any age. Once developed, it tends to persist life-long. Nickel is present in most of the dietary items and food is considered to be a major source of nickel exposure for the general population. Nickel in the diet of a nickel-sensitive person can provoke dermatitis. Careful selec...
Tissue engineering is a medical science dealing with reproduction of biological tissues and organs. This area of medicine opens avenues for creation of organs and tissues using biomaterials and nanostructures to sustain their development, maintenance and function repair in a living organism. The scope of tissue engineering is an artificial recreation of tissues at the fi nest structural level. Prerequisite requirements are a cell source (a donor), artificial extracellular matrix and growth facto...
#1Olga Wittig(IVIC: Venezuelan Institute for Scientific Research)H-Index: 6
#2Dylana Diaz-Solano(IVIC: Venezuelan Institute for Scientific Research)H-Index: 5
Last. Jose E. Cardier(IVIC: Venezuelan Institute for Scientific Research)H-Index: 17
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BACKGROUND Deep dermal and full-thickness burns are not only difficult to treat, but they are also associated with significant morbidity and mortality. Recent reports have proposed the use of mesenchymal stromal cells (MSCs) for inducing tissue repair in burn injuries. OBJECTIVE We aim to evaluate the effect of allogeneic MSC transplantation on full-thickness burns with delayed healing. MATERIAL AND METHODS This study includes five patients with AB B/B burns. All patients received conservative t...
Abstract Introduction Bi-layered skin reconstruction can be achieved by staged grafting of acellular dermal matrices (ADMs) and cultured epithelial keratinocyte sheets (KSs). Both KSs and ADMs have been used for long; yet, their combined use has shown poor effectiveness. This outcome has been related to the enzymatic treatment used in the preparation of KSs, which impairs their adhesion potential to ADMs and the formation of a basement membrane (BM). Temperature-responsive (TR) culture dishes al...
The use of additive manufacturing (AM) for the fabrication of biomedical implants has grown in the recent years because it gives the flexibility to create patient-specific customised implants and on-demand production. AM has opened the possibility of the fabrication of implants in the clinical setting, so-called bedside manufacturing that gives the opportunity to print directly into the patient or in a nearby location.
The purpose of wound healing is to repair the skin to prevent infection and to restore tissue integrity and function. Unfortunately, in adults, this process is geared toward faster rates of healing, to prevent infection, which ultimately leads to a compromise in the quality of healing. This compromise results in scarring, where the architecture of the skin is distinct from the original tissue, significantly affecting function and overall quality of life. The ideal for future treatments is to inc...
Abstract Modern biological and synthetic membranous wound dressings have improved treatment and outcome of partial-thickness burns. For full-thickness burns, the search for the ideal skin substitute is still ongoing. New developments are reviewed in this chapter, which comprise both materials and cell-seeded constructs. Cultured epidermal cells were used in burn treatment for decades, with moderate success. However, new culture and application techniques have revived the interest in this technol...
Last. Liam M. Grover(University of Birmingham)H-Index: 38
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Abstract This review gives an updated overview on keratinocyte transplantation in burn wounds concentrating on application methods and future therapeutic cell delivery options with a special interest in hydrogels and spray devices for cell delivery. To achieve faster re-epithelialisation of burn wounds, the original autologous keratinocyte culture and transplantation technique was introduced over 3 decades ago. Application types of keratinocytes transplantation have improved from cell sheets to ...
Mesenchymal stromal cells (MSCs) have shown to be a promising tool in cell therapies to treat different conditions. Several pre-clinical and clinical studies have proved that the transplantation of MSCs improves wound healing. Here, we compare the beneficial effects of mouse bone marrow-derived allogeneic MSCs (allo-mBM-MSCs) and their acelullar derivatives (allo-acd-mMSCs) on skin wound healing in Non-Obese Diabetic (NOD) mice. One dose of allo-mBM-MSCs (1×106 cells) or one dose of allo-acd-mMS...