Protein digestion optimization for characterization of drug–protein adducts using response surface modeling

The formation of drug–protein adducts in vivo may have important clinical and toxicological implications. Consequently, there is a great interest in the detection of these adducts and the elucidation of their role in the processes leading to adverse and idiosyncratic drug reactions. Enzymatic digestion is a crucial step in bottom-up proteomics strategies for the analysis of drug–protein adducts. The chosen proteolytic enzyme and digestion...