Simultaneous Determination of Five Constituents in Qinpijiegu Capsule by High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

A rapid high-performance liquid chromatography coupled with tandem mass spectrometry method was developed for the simultaneous determination of five constituents in Qinpijiegu capsule (QJC), a classical Tibetan prescription. The separation of five compounds such as aesculin, aesculetin, fraxin, peimine and peiminine was performed on a Purospher STAR LP RP-C18 (250 × 4.6 mm, 5 μm) column with linear gradient elution of acetonitrile–0.3‰ formic...