Engineering

Biology

Chromatography

Pig skin

Biochemistry

Human skin

Hairless

Food science

Genetics

Operations research

Internal medicine

Penetration (warfare)

Medicine

Chemistry

Guinea pig

Drug and Chemical Toxicology

The hairless guinea pig (HGP) is used by our laboratory to model the human cutaneous response to sulfur mustard (HD), bis(2-chloroethylsulfide), exposure. We determined the HD content in the skin of HGP after a 7-min exposure to vapors saturated with a mixture of HD and l4C-HD. Concentration/time (CT) values in the range of 2 μg/cm2/min were determined by counting skin 14C disintegrations per min (dpm) in animals euthanized immediately after exposure. These values are similar to human penetration rates obtained by other investigators. A rate curve monitoring the reduction in skin 14C dpm was developed for animals euthanized between 0 and 24 hr post- exposure. This curve showed the greatest change after 1 hr. The epidermal (62%) to dermal (38%) ratio of 14C at 24 hr was measured for two animals. We saw no site preference for HD penetration among the 8 sites used. The 14C content of template adhesive tape was determined to follow HD distribution. These results contribute to a better understanding of the cutaneous response to HD in the HGP model.

Cutaneous Uptake of<sup>14</sup>C-Hd Vapor By The Hairless Guinea Pig

Cutaneous Uptake of14C-Hd Vapor By The Hairless Guinea Pig

Cutaneous Uptake of¹⁴C-Hd Vapor By The Hairless Guinea Pig