The present experiments were undertaken to clarify the differences in humoral immune response to two β-lactam antibiotics, benzylpenicillin potassium (PcG) and cephalothin sodium (CET), in guinea-pig strains. Guinea pigs of three different strains, Hartley, Strain 2 and Strain 13, were immunized subcutaneously with PcG (10mg/body) 1 or 3 times a week, 10 times in total, or with CET (20 mg/body) 3 times a week, 12 times in total. Humoral immune responses to the two antibiotics were assessed by passive cutaneous anaphylaxis (PCA) and active systemic anaphylaxis (ASA) reactions. The relative intensities of the responses to PcG and CET detected by PCA reaction were Hartley > Strain 2 > Strain 13. On the other hand, Hartley and Strain 13 guinea pigs displayed high responses to the two antibiotics by ASA reaction, and Strain 2 exhibited a relatively low response. Based on these results, it was clarified that the Hartley strain, which is the most common strain used in ordinary allergenicity tests, showed the highest response to the two antibiotics tested in PCA and ASA reactions.
: The present experiments were undertaken to clarify the differences in humoral and cellular immune responses to a low-molecular compound, sodium 2,4,6-trinitrobenznnesul fonate dihydrate (TNBS) in guinea-pig strains. Guinea pigs of three different strains, Hartley, Strain 2 and Strain 13, were immunized subcutaneously with TNBS (3 mg/body) 2 or 3 times a week, 9 times in total. Humoral immune responses to TNBS were assessed by passive cutaneous anaphylaxis (PCA) and Arthus reaction, and cellula...
Optimal experimental methods for antigenicity studies in guinea pigs were investigated on : (1) the effects of different immunizing methods using complete or incomplete Freund's adjuvants (CFA or IFA), and various injection sites, the number of immunizations, the immunizing doses, and the immunizing periods, (2) the relationship between the severity of active systemic anaphylaxis (ASA) reactions and passive cutaneous anaphylaxis (PCA) titers, (3) positive control for oral administration, and (4)...
#1J.-C. Bidon(INRA: Institut national de la recherche agronomique)H-Index: 3
#2M. Blin(INRA: Institut national de la recherche agronomique)H-Index: 2
Last. A. Jondet(INRA: Institut national de la recherche agronomique)H-Index: 2
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The purpose of this study was to evaluate the strain-related differences in tracheal hyperresponsiveness in control and egg albumen-sensitized guinea pigs. Concentration-response curves to acetylcholi
#1François Verdier(French Institute of Health and Medical Research)H-Index: 13
#2Isabelle Chazal(French Institute of Health and Medical Research)H-Index: 1
Last. Jacques Descotes(French Institute of Health and Medical Research)H-Index: 30
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Abstract Immediate hypersensitivity (or anaphylactic) reactions can be caused by large molecules which are directly immunogenic or by smaller molecules which bind to macromolecular carriers and act as haptens. To reproduce these reactions in animals, either systemic or local models are used in actively or passively sensitized animals, respectively. Several attempts have been made to detect the potential of new drugs and chemicals to induce anaphylactic reactions. Protocols using the inhalation o...
In order to develop a system for evaluating the allergenicity of drugs in clinical use, we tested drugs for the ability to induce drug-specific immune responses in guinea pigs and mice. Test drugs were benzylpenicillin, procainamide, hydralazine, isoniazid, α-methyldopa, D-penicillamine, captopril, sulfamethoxazole and 2, 4-dinitrochlorobenzene (DNCB), which are known to induce allergic responses in man including hypersensitivity reactions and drug-induced auto-immune responses. Guinea pigs were...
Development of eosinophilia was studied in four strains of guinea pigs (gp), selectively bred for either high or low respiratory anaphylactic reactivity. One high-asthma strain (IMM/S 209) and one low-asthma strain (IMM/R 203) developed spontaneous high blood eosinophilia. The 2 other gp strains - one high-asthma strain (IMM/S 740) and one low-asthma strain (IMM/R 201–16) – maintained normal low levels of eosinophilic granulocytes (eos). The levels of eos in various tissues showed similar differ...
The present studies were undertaken to clarify the immunogenicity evoked in four species, the guinea pig, rabbit, F344 rat and A/J mouse, immunized with a low-molecular antigen (TNBS) alone, TNBS plus Alum and TNBS plus FCA. Each species was divided into three groups: TNBS alone sc group, TNBS plus Alum ip groups (one and 3 times sensitization) and TNBS plus FCA sc groups (one and 3 times sensitization), and immune responses to TNBS were estimated by the assay systems of humoral immunoreactions ...
The present study was carried out using a low-molecular antigen to clarify which of the two IgG subclasses (IgG1 and IgG2) of guinea pigs is responsible for the immunological assay systems in ordinary antigenicity tests for the development of a novel drug, and to what extent such IgG subclass antibodies contribute to the assay systems in addition to IgM antibody. Guinea pigs were immunized with TNBS plus FCA (3 mg/body), at intervals of 10 days, 3 times in total. The anti-TNBS serum was fraction...
: It appears to be significant for the analytical studies of drug allergy that a comparison is made among inbred strains of mice, with different H-2 haplotypes with respect to the antibody response to low-molecular compounds. Such studies have been demonstrated by several investigators using high-molecular antigens, such as the synthetic polypeptides and natural proteins. The present experiments were undertaken, therefore, to clarify the differences in antibody responses to a low-molecular compo...
We have examined the immunological activity of five affinity-purified protein antigens from Mycobacterium tuberculosis in seven inbred and one outbred guinea pig strains. The test systems were measurements of delayed-type hypersensitivity (Dth) responses, lymphocyte stimulation assays (LS), and antibody response measurements. The results showed significant differences in the immunogenicity of the single-protein antigens and, when the antigens were considered separately, highly significant guinea...
Purpose We previously reported the safety and efficacy in animal experiments of transcutaneous immunization (TCI) using a self-dissolving microneedle patch (MicroHyala; MH) made of hyaluronic acid and collagen. However, this MH was an unsuitable TCI device for the human skin, as collagen is suspected to induce inflammation. In this study, we developed an improved collagen-free MH (new-MH) and conducted clinical study to evaluate the fundamental properties and safety in human.
In the present study, the correlation between mouse PLNA and those obtained from GP-PCA and from GP-ASA reactions fro sodium 2, 4, 6-trinitrobenzenesulfonate dihydrate (TNBS), penicillin G and cephalothin was investigated. Next, variou drugs were tested using the mouse PLNA to study whether PLN reactivity could be related to the potential of the compounds to induce allergic and autoimmune disorders in humans. The parameter of the PLNA was determined by the PLN cellularity index in BALB/c and A/J...