Comprehensive two-dimensional liquid chromatography coupled with quadrupole time-of-flight mass spectrometry for chemical constituents analysis of tripterygium glycosides tablets

Comprehensive two-dimensional liquid chromatography platform (LC×LC) coupled with quadrupole time-of-flight (QTOF) mass spectrometry (MS) is developed to separate, identify and relatively determine the chemical constituents of two types of tripterygium glycosides tablets (TGT). The types and relative contents of the constituents discovered in two kinds of TGT tablets were subsequently compared. C8 and C18 column were used for the separation of...