N-Glycan Profiling by Microchip Electrophoresis to Differentiate Disease States Related to Esophageal Adenocarcinoma
Abstract
We report analysis of N-glycans derived from disease-free individuals and patients with Barrett's esophagus, high-grade dysplasia, and esophageal adenocarcinoma by microchip electrophoresis with laser-induced fluorescence detection. Serum samples in 10 μL aliquots are enzymatically treated to cleave the N-glycans that are subsequently reacted with 8-aminopyrene-1,3,6-trisulfonic acid to add charge and a fluorescent label. Separations at 1250...
Paper Details
Title
N-Glycan Profiling by Microchip Electrophoresis to Differentiate Disease States Related to Esophageal Adenocarcinoma
Published Date
Apr 3, 2012
Journal
Volume
84
Issue
8
Pages
3621 - 3627
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