Nutritional repletion of malnourished tumor-bearing and nontumor-bearing rats: Effects on body weight, liver, muscle, and tumor☆

Published on Jun 1, 1980in Journal of Surgical Research1.841
· DOI :10.1016/0022-4804(80)90043-8
John M. Daly66
Estimated H-index: 66
(University of Texas System),
Edward M. Copeland77
Estimated H-index: 77
(University of Texas System)
+ 1 AuthorsJohn M. Delaney1
Estimated H-index: 1
(University of Texas System)
Sources
Abstract
Effects of nutritional repletion were evaluated in malnourished rats with either no tumor (NTB), small tumor burdens (TB), or large tumor burdens (TB). One hundred and four Sprague-Dawley rats were inoculated with Walker-256 carcinosarcoma and were fed regular diet (RD) for 5 days in Study A. At this time, one group was maintained on RD while the remaining rats were fed a high carbohydrate, protein-free diet (PFD). On Day 15, one PFD group was switched to RD; one PFD group continued on PFD. Eighty-nine NTB rats received an identical dietary protocol. Animals were killed on Days 15, 21, and 33. Mean food intake and carcass weight changes were similar in corresponding groups of TB and NTB rats in Study A. After Day 15, mean carcass weight and liver and muscle protein content increased rapidly in PFD → RD TB and NTB rats, becoming similar to the RD group by Day 33. By Day 33, mean tumor weights and mean total tumor protein content in the PFD group were significantly less than the RD or PFD → RD groups. In Study B, tumor-bearing animals were fed regular diet for 20 days after tumor inoculation to produce a larger tumor burden prior to nutritional depletion. Twelve rats were given PFD; six rats continued on the RD. On Day 30, one PFD group (n = 6) was switched to RD while the other PFD group (n = 6) continued on PFD. On Day 36, all rats were sacrificed. Mean carcass weight, liver, and muscle protein levels at sacrifice were significantly less in the PFD → RD group compared with the RD group, but mean serum protein levels were similar in the two groups. Animals co$tinued on PFD lost significant carcass weight, liver, and muscle protein. The presence of a small tumor (5% carcass weight) did not affect protein repletion in host liver or muscle when an adequate diet was provided, although tumor growth was increased. The presence of a larger tumor burden (>25% carcass weight) significantly inhibited host nutritional repletion.
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The effects of nutritional manipulation on host body weight dynamics, tumor growth patterns and host-tumor responses to chemotherapy were studied in Sprague-Dawley rats with Walker-256 carcinosarcomas. Group I maintained throughout on a regular diet (RD) gained carcass weight steadily. Group II lost carcass weight while fed a protein-free diet (PFD) but rapidly gained weight after switching to RD on day 15. Mean tumor volume increased 105% in Group I from day 15 to 21, 218% in Group II and 77% i...
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The data presented in this paper demonstrate that a liver which has been severely damaged by protein malnutrition can be restored to normal composition, anatomy and functional capability by central venous feeding. An atypical serum protein electrophoretic pattern characterizes protein malnutrition. TPN repletion restores all of the protein fractions back to normal except for the gamma globulin levels. Liver nitrogen is a very sensitive index of both protein malnutrition and TPN repletion. The li...
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BUF rats with a transplantable Morris hepatoma No. 7777 were given three feeding regimens, (i) solid food ad libitum, (ii) total parenteral hyperalimentation, and (iii) liquid diet ad libitum. Those rats on solid or liquid food ad libitum undergo body weight loss or cancer cachexia with decreased food intake. Total parenteral hyperalimentation prevented the loss in body weight but stimulated tumor growth when compared to those fed ad libitum. Although there was no significant difference in survi...
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The difficulty of maintaining adequate nutritional status in cancer patients is well recognized [I, 181. Recently there has been considerable interest in the clinical use of intravenous diets to support these patients [2, 51. Although present evidence strongly suggests that the cancer patient receiving chemoor radiotherapy is significantly benefited by parenteral nutrition, there exists little information on the metabolic changes induced in tumor and host tissue by parenteral nutrition. Unfortun...
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Since the introduction of intravenous hyperalimentation (IVH) as a nutritional adjunct in multimodal cancer therapy (X), the risk of providing nutrient substrates for more rapid tumor growth has been a concern of those who use IVH to rehabilitate malnourished cancer patients nutritionally. Because of the technical difficulties in studying glucose and amino acid utilization in cancer patients, an experimental model was designed to simulate the nutritional problems encountered in cachectic cancer ...
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