Anti-inflammatory activity of Devil’s claw in vitro systems and their active constituents

Cell suspension and Agrobacterium rhizogenes-transformed hairy root cultures of Devil’s claw (Harpagophytum procumbens), an African plant with high medicinal value, were cultivated in shake-flasks. A purification scheme to isolate their main active constituents (the phenylethanoid glycosides verbascoside, leucosceptoside A, β-OH-verbascoside and martynoside; structurally identified by NMR and LC–MS) was then developed, and their concentrations...